The screening platform is a crucial component of DNA-encoded libraries (DELs) screening services. It involves a series of sophisticated technologies and methodologies designed to efficiently and accurately identify active compounds from vast chemical libraries. Here, we listed the key elements and technologies involved in the DELs screening platform:
Automated Screening Systems
High-Throughput Screening (HTS)
Our HTS platforms are equipped with advanced automation and detection capabilities, enabling researchers to efficiently screen large compound libraries against diverse molecular targets.
Microfluidic Screening: Microfluidic Devices
- Function: Microfluidic platforms utilize small channels and chambers to manipulate tiny volumes of liquids, enabling high-precision assays.
- Process: Compounds are screened in microfluidic chips that allow for precise control of assay conditions and reagent mixing.
- Benefits: Reduced reagent consumption, increased assay sensitivity, and the ability to perform multiple assays simultaneously.
Binding Assay Technologies
Surface Plasmon Resonance (SPR): SPR Sensors
- Function: SPR technology measures the binding interactions between compounds and target proteins in real-time without the need for labeling.
- Process: Compounds are immobilized on a sensor chip, and the binding of target molecules is detected by changes in the refractive index at the sensor surface.
- Benefits: High sensitivity, real-time data acquisition, and quantitative analysis of binding kinetics.
Fluorescence Polarization (FP): FP Assays
- Function: FP measures the binding interactions between small fluorescently-labeled molecules and larger target proteins based on changes in rotational speed.
- Process: The binding of a small, fluorescent ligand to a larger target protein increases the molecule's rotational correlation time, detectable by changes in fluorescence polarization.
- Benefits: Simple, homogeneous assay format, suitable for high-throughput screening.
Selection Systems
Affinity Selection: Magnetic Bead-Based Separation
- Function: Magnetic beads coated with target proteins capture and isolate binding compounds from the library.
- Process: Compounds that bind to the target proteins are separated using a magnetic field, allowing non-binding compounds to be washed away.
- Benefits: High specificity, scalability, and compatibility with various assay formats.
Competitive Elution: Competitive Binding Assays
- Function: Competitive elution assays identify compounds that bind to the target by competing with a known ligand.
- Process: A known ligand is used to elute binding compounds from the target protein, allowing for the identification of high-affinity binders.
- Benefits: Effective for identifying competitive inhibitors and measuring binding affinities.
Sequencing and Data Analysis
High-Throughput Sequencing (HTS): Next-Generation Sequencing (NGS)
- Function: NGS technologies decode the DNA tags on DELs compounds, identifying active compounds with high precision.
- Process: DNA tags are amplified and sequenced, providing comprehensive data on the library's composition and the identity of hits.
- Benefits: High throughput, accuracy, and the ability to handle large datasets.
Bioinformatics and Data Analysis: Advanced Bioinformatics Tools
- Function: Bioinformatics platforms analyze sequencing data, identifying active compounds and mapping their chemical structures.
- Process: Data from NGS is processed using algorithms and machine learning techniques to predict biological activity and optimize hit identification.
- Benefits: Enhanced data interpretation, integration with other drug discovery processes, and improved hit validation.
Automated screening systems, sophisticated binding assays, precise selection techniques, and robust sequencing and data analysis platforms collectively ensure the successful identification of bioactive compounds. Partner with us for unparalleled quality and service in advancing your scientific endeavors.