In the realm of drug discovery, the transition from hit identification to lead optimization is a critical phase that demands precise and detailed characterization of molecular interactions. Biophysical assays are at the forefront of this process, providing invaluable data on binding affinities, kinetic parameters, and thermodynamic properties. Alfa Chemistry is equipped with state-of-the-art biophysical technologies, ensuring that each step of hit-to-lead optimization is supported by robust and high-quality data.
Our Services
At Alfa Chemistry, we are committed to excellence in biophysical assay services, including:
Surface Plasmon Resonance (SPR)
Surface Plasmon Resonance (SPR) is a highly sensitive, label-free technique that allows real-time monitoring of biomolecular interactions. By leveraging SPR, Alfa Chemistry can identify the most promising candidates early in the drug discovery process, optimizing them through iterative cycles of testing and refinement.
- High Sensitivity and Throughput: The SPR systems are designed to handle high-throughput screening with remarkable sensitivity. This enables the rapid validation of hits and the detailed kinetic characterization of lead compounds.
- Comprehensive Data Analysis: SPR provides both kinetic (association and dissociation rate constants) and equilibrium (dissociation constant, KD) data in a single run, facilitating a comprehensive understanding of molecular interactions.
BioLayer Interferometry (BLI)
BioLayer Interferometry (BLI) is another powerful technique used by Alfa Chemistry for real-time analysis of biomolecular interactions. Utilizing the Octet RED384 system, BLI offers high-throughput capabilities and versatility.
- Versatility: The BLI system can simultaneously monitor multiple interactions under various conditions, including different concentrations, antibodies, and inhibitors. This versatility accelerates the screening and optimization process.
- Enhanced Efficiency: With sixteen biosensors, the system can process numerous samples simultaneously, significantly reducing the time required for comprehensive interaction analysis.
Temperature Related Intensity Change (TRIC)
Temperature Related Intensity Change (TRIC) is a cutting-edge technique that measures changes in fluorescence intensity upon laser-induced heating. Alfa Chemistry employs the Dianthus NT.23Pico system for rapid and accurate affinity assessments.
- Flexible Sample Compatibility: TRIC can handle a wide range of sample types, including aqueous solutions, plasma, and cell lysates, making it a versatile tool for different stages of drug discovery.
- Facilitating Lead Optimization: By delivering easy-to-interpret data, TRIC helps in making informed decisions about candidate prioritization and optimization.
Thermal Shift Assay (TSA)
Thermal Shift Assay (TSA) is utilized to assess the thermal stability of target proteins in the presence of potential ligands. This technique provides insights into the binding interactions and stabilizing effects of compounds.
- Melting Point Shifts: TSA measures the shift in the melting point (Tm) of proteins, indicating how ligands affect protein stability.
- High-Throughput Capability: Alfa Chemistry's TSA platforms support both 96-well and 384-well formats, allowing for large-scale screening and rapid data generation.
Isothermal Titration Calorimetry (ITC)
Isothermal Titration Calorimetry (ITC) directly measures the heat changes associated with binding events, providing detailed thermodynamic profiles of interactions. Alfa Chemistry uses the MicroCal PEAQ-ITC system for these analyses.
- Comprehensive Thermodynamic Data: ITC delivers key parameters such as binding affinity (KD), stoichiometry (n), enthalpy (ΔH), and entropy (ΔS) in a single experiment.
- Precision and Sensitivity: The MicroCal PEAQ-ITC system is highly sensitive and requires low sample volumes, making it ideal for studying delicate and low-abundance interactions.
Our Strengths
- Cutting-Edge Technology
Alfa Chemistry's investment in state-of-the-art biophysical instruments ensures the generation of high-quality data.
- Expertise and Customization
Our team of experts excels in interpreting biophysical data and providing customized guidance for hit-to-lead optimization.
- High Throughput and Versatility
The high-throughput capabilities of our instruments enable rapid screening and detailed analysis, significantly accelerating the drug discovery process.
Through the strategic application of SPR, BLI, TRIC, TSA, and ITC, Alfa Chemistry delivers robust support in the development of novel therapeutics, driving efficiency and success in drug discovery. Contact us today to learn more about our services and discover how we can empower your journey towards innovation and success.