Biochemical assays are integral to the drug discovery process, providing critical insights into biomolecular functions. These assays are essential in the early stages of drug development, particularly in target characterization, hit validation, and hit-to-lead optimization. At Alfa Chemistry, we leverage our extensive expertise and advanced technologies to deliver high-quality biochemical assay services tailored to the specific needs of our clients.
Our Services
At Alfa Chemistry, we are committed to excellence in biochemical assay services, including:
Comprehensive Biochemical Assay Development
At Alfa Chemistry, we specialize in the development of functional biochemical assays across a diverse range of target classes. Our services include:
- Kinase Assays: Critical for identifying inhibitors that regulate kinase activity, our kinase assays utilize both fluorescence-based and luminescence-based techniques to measure enzymatic activity.
- Hydrolase Assays: Employing colorimetric and fluorometric methods, we assess the activity of hydrolases, which play a pivotal role in metabolic processes.
- Protease Assays: Using substrates that generate a detectable signal upon cleavage, our protease assays help identify inhibitors with therapeutic potential.
- Deubiquitinylating Enzymes (DUBs) Assays: We provide assays to screen for modulators of DUB activity, which are implicated in various diseases including cancer and neurodegeneration.
- Phosphatase Assays: These assays are designed to identify compounds that modulate phosphatase activity, utilizing advanced techniques such as mass spectrometry.
- Dehydrogenase Assays: We offer robust assays for the detection of dehydrogenase activity, crucial for studying metabolic pathways.
- Transferase Assays: Our transferase assays utilize substrates that produce measurable changes upon transfer of functional groups, aiding in the discovery of transferase inhibitors.
- Ligase Assays: We develop ligase assays to screen for inhibitors that interfere with the ligation process, important in DNA repair mechanisms.
- DNA/RNA Polymerase Assays: These assays are essential for identifying inhibitors of polymerase enzymes, which are targets for antiviral and anticancer therapies.
- Protein-Protein Interaction Assays: Using techniques such as co-immunoprecipitation and surface plasmon resonance, we study the interactions between proteins.
- Protein-DNA/RNA Interaction Assays: Our assays explore the binding interactions between proteins and nucleic acids, using electrophoretic mobility shift assays and chromatin immunoprecipitation.
Advanced Detection Methods
Our state-of-the-art detection methods ensure precise and accurate measurement of biochemical activities:
- Colorimetric Assays: Utilizing absorbance measurements, these assays provide quantitative data on enzymatic activity.
- Fluorometric Assays: Techniques such as Fluorescence Polarization (FP), Förster Resonance Energy Transfer (FRET), and calcium flux assays offer high sensitivity and specificity.
- Luminescent Assays: Including Enzyme-Linked Immunosorbent Assay (ELISA), Alpha Technology, Bioluminescence Resonance Energy Transfer (BRET), and Nano-BRET, these assays enable the detection of low-abundance targets with high sensitivity.
- Gel-Based Assays: Techniques such as Western Blotting (WB) and pulldown assays provide qualitative and semi-quantitative analysis of protein interactions and modifications.
Our Strengths
- Customization and Flexibility
We recognize that each drug discovery project is unique, requiring tailored approaches to meet specific needs. Alfa Chemistry offers highly customizable assay development services, allowing us to design and optimize assays that align with our clients' research objectives.
- Cutting-Edge Instrumentation
From automated liquid handling systems to high-resolution mass spectrometers, our state-of-the-art equipment ensures the highest standards of data quality.
- Commitment to Quality
Quality is at the core of our operations. We adhere to stringent quality control measures throughout the assay development process, from initial design to final data analysis.
Our comprehensive services, advanced technologies, and commitment to quality ensure that we provide reliable, high-quality data to support the progression of therapeutic candidates. Contact us today to learn more about our services and discover how we can empower your journey towards innovation and success.