Drug research and development has witnessed a new upsurge with the development of DNA-encoded libraries (DELs) and DELs technology, which has improved the efficiency of screening lead compounds. DELs construction requires a high-level supply of DELs primers whose quality directly affects subsequent library production and selection. A primer refers to a short single-stranded nucleic acid that provides an initiation point for DNA synthesis. A polymerase chain reaction (PCR) uses them to determine the region of DNA that will be copied or amplified.
Alfa Chemistry provides high-quality DELs primer synthesis services to help researchers successfully construct DELs. In order to advance your research and applications, we are committed to breaking down the barriers between chemical synthetic primers and their biological applications. Not only do we have an expert team with many years of experience in the field of primer synthesis, we have also developed a complete quality inspection system based on the process and principles of DELs construction.
Primers for DELs
Alfa Chemistry provides custom synthesis services of primers that can be annealed to specific sites on the templated DNA. In solution, primers spontaneously hybridize to the template via Watson-Crick base pairing and are extended by DNA polymerase. Primers are required to initiate the reaction during subsequent next-generation sequencing of DNA tags. The core primers required for DELs construction are listed below.
- Headpiece, AOP-HP and AOP-HP-primer for connecting small molecules and DNA barcodes.
- DNA tags that are used to encode small molecules.
- Closing primers that are used for sequencing and decoding the correspondence between DNA tags and small molecules.
Key Features of Primers
During the design and synthesis of DELs primers, it is necessary to pay attention to their conventional quality control (QC) indicators such as base error rate, purity, and quantification. Furthermore, the rate of phosphorylation modification needs to be considered. Since DELs primers are connected to each other through T4 ligases, the 5' terminal phosphate group is required to form a phosphodiester bond. It is worth noting that the phosphorylation efficiency of DELs primers also greatly affects the efficiency of DELs construction. Listed below are several key features of the primers provided by Alfa Chemistry for compound library construction.
Our Services
The following table lists the primer synthesis items provided by Alfa Chemistry. Please feel free to contact us if you have any questions.
| Service Items | Purification method | Modification | Base range | Primer specifications | Delivery form |
| Primer Synthesis | HPLC | 5' Phosphorylation | 5-60 nt | Inquiry |
|
| DSL |
Choose Us
Alfa Chemistry is committed to providing primer synthesis services for DELs construction. We have a high-throughput, large-scale synthesis platform that can meet various needs for library construction. Our strengths include:
Precise quantification
We have a self-developed primer quantitative calculation formula, a fully automatic quantitative dispensing workstation and enzyme labels for precise quantitative combinations to ensure accurate quantification.
High enzymatic ligation efficiency
We guarantee that the primers used in library construction for enzymatic ligations have high ligation efficiency, and the stability of each modified primer is >95%.
Near zero error rate of base
We strive to create library construction primers with zero base error rate to further ensure the precise identification of hits.
Standardized DNA synthesis process
We perform a standardized primer synthesis process to reduce batch-to-batch differences and ensure stable batch quality.
Various purification methods
According to different DELs construction needs, different purification options such as desalination (DSL) and high-performance liquid chromatography (HPLC) can be flexibly provided.
Fast delivery
Our synthesis cycle is short and delivery speed is fast, which can effectively save you time.
Strict QC
We perform high-precision liquid chromatography–mass spectrometry (LC–MS) analysis for each primer to ensure its purity and accuracy, and provide a high-precision MS analysis report for each primer.
Order Process
