Fluorolube grease,gr-362

• CAS: 9002-83-9
• Catalog Number: ACM9002839
• Category: Main Products
• Molecular Formula: C₄Cl₄F₆
• Synonyms: HALOCARBON OIL;HALOCARBON OIL 27;HALOCARBON OIL 700;FLUOROLUBE GREASE, GR-290;FLUOROLUBE GREASE, GR-362;FLUOROLUBER GREASE GR-362;FLUOROLUBE (TM) GREASE GR-362 (R);POLY(CHLOROTRIFLUOROETHYLENE)
• IUPAC Name: Ethene, 1-​chloro-​1,​2,​2-​trifluoro-​, homopolymer
• Appearance: White powder
• Exact Mass: 115.96400

Case Study

Halocarbon Oil 27 Used for Advanced Live Imaging of Drosophila Egg Chambers

Prasad, Mohit, et al. Nature protocols 2.10 (2007): 2467-2473.

Studying specific developmental stages in Drosophila melanogaster, particularly stage 9 egg chambers, historically faced significant limitations. Existing culture methods failed to maintain normal development beyond brief periods, especially for processes like border cell migration. This study details a protocol that enables continuous in vitro culture and live imaging of stage 9 egg chambers, in which halocarbon oil 27 plays an integral role in preventing evaporation.
Key Findings
· Non-Negotiable Role of Halocarbon Oil 27: Omitting halocarbon oil 27 resulted in evaporation of the culture medium, completely preventing normal egg chamber development and border cell migration.
· Precision-Dependent Efficacy: Applying a very thin layer of halocarbon oil 27 effectively minimized evaporation without disrupting imaging. Excess oil caused coverslip flotation and sample movement.
· Troubleshooting Validation: Excess oil or medium (Steps 13/17) directly caused sample movement, resolved only by removing surplus oil/medium and reapplying a minimal oil layer.
· This work established a reliable in vitro culture and live imaging protocol for stage 9 egg chambers that supports normal development (including border cell migration) for 24 hours, enabling molecular studies and pharmacological testing.