Fluorescent Brightener 28

• CAS: 4193-55-9
• Catalog Number: ACM4193559
• Category: Main Products
• Molecular Weight: 960.95
• Molecular Formula: C40H44N12O10S2.2Na
• Synonyms: Calcofluor White ST
• Appearance: Yellow-greenish powder

Case Study

Synthesis of Fluorescent Brightener 28-Coated Fe3O4 Nanoparticles

Tegafaw T, et al. Journal of Nanoscience and Nanotechnology, 2016, 16(10), 10986-10990.

Fe3O4 nanoparticles are coated with fluorescent brightener agent 28 (FB28), which exhibits strong photoluminescence (PL) emission at 435 nm and has potential for biomedical applications. Furthermore, they also provided confocal images of strong fluorescence in the green and blue regions due to the nanoparticles being coated with FB 28.
Synthesis process of FB28-coated Fe3O4 nanoparticles
· Firstly, almost monodispersed Fe3O4 nanoparticles were produced in the aqueous phase from FeCl2·4H2O.
· FB 28 (0.2 mmol) was dissolved in triply distilled water (150 mL) in a 300 mL three-necked flask at room temperature under an argon flow. The mixture was mechanically stirred until the FB 28 was completely dissolved.
· The synthesized nanoparticles were then added to the FB 28 solution and the solution was mechanically stirred for 8 h. The solution containing the product was poured into a beaker containing triply distilled water (400 mL). The solution was stirred for 10 min and stored to allow the product nanoparticles to settle to the bottom of the beaker.

In vivo detection of chitin in butterfly wings using fluorescent brightener 28

Nakazato, Yugo, and Joji M. Otaki Insects 14.9 (2023): 753.

Chitin, a major component of the extracellular cuticle, plays a variety of roles in insects. In butterflies, chitin is involved in the formation of scales and color patterns. Fluorescent brightener 28 (FB28) was used to detect intracellular chitin in living cells, focusing on the wing epithelium of a small, grey butterfly. Strongly FB28-positive cells were observed in the cytoplasm, suggesting that these cells are specialized for chitin secretion. Using FB28 to detect intracellular chitin in living cells may also be applicable to other insect systems.
Fluorescent brightener 28 and other fluorescent probes were topically applied to the wing epithelium using a sandwich method. Immediately after pupation, the left forewing was lifted with forceps under a stereomicroscope. A 4 μL droplet containing fluorescent brightener 28 and other fluorescent probes was applied to the surface of the dorsal wing and sandwiched between the ventral forewing and dorsal wing. After incubation for 1 to 2 hours, the wing tissue surface was washed with phosphate-buffered saline (PBS). Place the ventral forewings and dorsal wing surfaces on a thin glass plate and cover with plastic wrap to prevent evaporation. Examine the ventral forewings under a fluorescence confocal microscope. This should begin within 20 minutes of pupation.

Study on Fe₃O₄ nanoparticles coated with fluorescent brightener 28 (FB-28)

Tegafaw, Tirusew, et al. Journal of Nanoscience and Nanotechnology 16.10 (2016): 10986-10990.

Nearly monodisperse Fe₃O₄ nanoparticles were synthesized in aqueous solution under an argon flow and then coated with fluorescent brightener 28 (FB-28). The Fe₃O₄ nanoparticles exhibit superparamagnetism, with a large saturation magnetization of 54.1 emu/g at 5 K and 48.5 emu/g at 300 K. Due to the FB-28 coating on the nanoparticles, the FB-28-coated Fe₃O₄ nanoparticles exhibit strong fluorescence maxima in the green and blue regions and also give strong fluorescence confocal images. These magnetic and optical properties are expected to be useful in various biomedical applications.
For surface coating, fluorescent brightener 28 was dissolved in 150 mL of triple-distilled water in a 300 mL three-necked flask at room temperature under an argon flow. The mixture was mechanically stirred until the fluorescent brightener 28 was completely dissolved. The synthesized nanoparticles were then added to the FB 28 solution, and the solution was mechanically stirred for 8 hours. The resulting solution was poured into a beaker filled with 400 mL of triple-distilled water. The solution was stirred for 10 minutes and then stored to allow the product nanoparticles to settle to the bottom of the beaker.

The effects of Fluorescent Brightener 28 on the growth and morphology of red algae

Belliveau, Daniel J., David J. Garbary, and Jack L. McLachlan. Stain technology 65.6 (1990): 303-311.

The binding affinity, toxicity, and teratogenicity of four fluorescent brighteners (Fluorescent Brightener 28, Fluostain 1, Fluostain, and Cellufluor) on the red alga Anfifhmnion kylinii were investigated. FB-28 exhibited the greatest growth inhibition of the stains. Filaments incubated at a low stain concentration (0.0005%) exhibited cellular abnormalities in all stain types. The results suggest that extensive experimentation is required to develop protocols for key cell wall stains that minimize toxicity and maximize binding.
A 0.01% solution of Fluorescent Brightener 28 was used for staining. Ten apical segments, 1.5-3.0 mm in length, were cultured with each stain and in a control culture containing no brightener. The relative fluorescence of two experimental and two control filaments was measured immediately after staining (day 0) and at days 2, 4, 7, 8, and 12. On day 0, fluorescence was measured between the two lateral pit junctions, where cell elongation was not occurring. On days 2, 4, 8, and 12, fluorescence was measured in both non-extending and elongating regions of the cells. The lower the B/A binding ratio, the stronger the binding of the stain.

Fluorescent Brightener 28 used for flow cytometric staining

Davey, Hazel M., and Douglas B. Kell. Cytometry: The Journal of the International Society for Analytical Cytology 28.4 (1997): 311-315.

Flow cytometry is a rapid method for measuring the optical properties of single cells. The technique has found great utility in the study of mammalian cells, but its applications in microbiology are more limited. Fluorescent Brightener 28 and other dyes are effective stains for vegetative microbial cells and spores of Gram-positive bacteria. Pre-treating the sample with ethanol accelerates the staining process. Under favorable conditions, the dye can be used to distinguish between organisms, a fact that can be useful when screening for target microorganisms against a high biological background.
Fluorescence measurements were performed using a spectrofluorometer. Water samples containing Fluorescent Brightener 28 and other dyes were placed in 3 ml cuvettes (1 cm light path) for analysis. An excitation wavelength of 325 nm was selected because it is a UV wavelength available in flow cytometers. Emission scans were performed by measuring the intensity of emitted light at 1 nm wavelength intervals between 300 and 800 nm.

Custom Q&A

What is the PubChem CID of Fluorescent brightener 28?

The PubChem CID of Fluorescent brightener 28 is 6434593.

What is the molecular formula of Fluorescent brightener 28?

The molecular formula of Fluorescent brightener 28 is C40H42N12Na2O10S2.

What are the synonyms of Fluorescent brightener 28?

The synonyms of Fluorescent brightener 28 are Cellufluor, Calcofluor White ST, Calcofluor White LRP, and more.

What is the molecular weight of Fluorescent brightener 28?

The molecular weight of Fluorescent brightener 28 is 960.9 g/mol.

What is the parent compound of Fluorescent brightener 28?

The parent compound of Fluorescent brightener 28 is C.I. Fluorescent brightening agent 28 (CID 6108780).

What are the component compounds of Fluorescent brightener 28?

The component compounds of Fluorescent brightener 28 are Sodium (CID 5360545) and C.I. Fluorescent brightening agent 28 (CID 6108780).

When was Fluorescent brightener 28 created?

Fluorescent brightener 28 was created on April 28, 2006.

When was Fluorescent brightener 28 last modified?

Fluorescent brightener 28 was last modified on December 30, 2023.

What is the IUPAC name of Fluorescent brightener 28?

The IUPAC name of Fluorescent brightener 28 is disodium;5-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-[(E)-2-[4-[[4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-2-sulfonatophenyl]ethenyl]benzenesulfonate.

What is the CAS number of Fluorescent brightener 28?

The CAS number of Fluorescent brightener 28 is 4193-55-9.

What is the InChI of Fluorescent brightener 28?

The InChI of Fluorescent brightener 28 is InChI=1S/C40H44N12O10S2.2Na/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56;;/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50);;/q;2*+1/p-2/b12-11+;;

What is the Canonical SMILES of Fluorescent brightener 28?

The Canonical SMILES of Fluorescent brightener 28 is C1=CC=C(C=C1)NC2=NC(=NC(=N2)N(CCO)CCO)NC3=CC(=C(C=C3)C=CC4=C(C=C(C=C4)NC5=NC(=NC(=N5)NC6=CC=CC=C6)N(CCO)CCO)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]

What is the UNII of Fluorescent brightener 28?

The UNII of Fluorescent brightener 28 is 11V30J47QK.

What is the hydrogen bond donor count of Fluorescent brightener 28?

The hydrogen bond donor count of Fluorescent brightener 28 is 8.

What is the hydrogen bond acceptor count of Fluorescent brightener 28?

The hydrogen bond acceptor count of Fluorescent brightener 28 is 22.

What is the rotatable bond count of Fluorescent brightener 28?

The rotatable bond count of Fluorescent brightener 28 is 20.