Propamidine,75%

• CAS: 104-32-5
• Catalog Number: ALC-FP-104325
• Category: Featured Products
• Molecular Weight: 312.37
• Molecular Formula: C17H20N4O2
• Appearance: White to yellow powder
• Sample Lot No.: A24C523E2

Case Study

Liposome-Mediated Intracellular Delivery of Propamidine for Macrophage Apoptosis

N van Rooijen, et al. Journal of Immunological Methods, 1996, 193(1), 93-99.

In this study, propamidine was encapsulated in liposomes to investigate its intracellular effects on phagocytic cells. Peritoneal macrophages and RAW 264 macrophage cell lines were cultured in the presence of liposome-encapsulated propamidine, alongside clodronate and EDTA as comparators. The liposomal formulation facilitated efficient uptake through phagocytosis, resulting in intracellular accumulation of propamidine. Subsequent metabolic disruption triggered apoptotic cell death, confirmed by morphological and biochemical evidence of apoptosis. While EDTA showed minimal apoptotic induction, liposomal propamidine demonstrated robust efficacy in depleting macrophages. This experimental approach highlights the utility of liposome-mediated delivery in directing small-molecule drugs into phagocytic cells, establishing propamidine as an effective tool in macrophage depletion studies.