Lactose

• CAS: 63-42-3
• Catalog Number: ALC-FP-63423
• Category: Featured Products
• Ash Content: ≤ 0.30%
• As Is Protein: ≤ 0.30(Nx6.38, %)
• Coliforms: < 10(cfu/g)
• E. Coli: < 10(cfu/g)
• Lactose: ≥ 99.0%
• Moisture Content: ≤ 0.50%
• Mold: < 50(cfu/g)
• Munsell Color: ≤ 2.5
• pH: 4.5-7.5
• Salmonella: Negative (/375g)
• Sample Lot No.: A23GY0403X
• Scorched Particle: ≤ 15.0 (mg/25g)
• Staphylococcus Aureus: < 10(cfu/g)
• Yeast: < 50(cfu/g)

Case Study

Lactose Utilization for the Construction of a Lactose-Metabolizing Pseudomonas putida KT2440

Saumaa S, et al. Journal of Biotechnology, 2025, 406, 225-235.

This case study describes the experimental engineering of Pseudomonas putida KT2440 to enable lactose utilization as a sole carbon source. Lactose was applied as a selective substrate in minimal media to drive pathway optimization. Experimentally, plasmid-based expression of lacZ enabled intracellular lactose hydrolysis, while chromosomal integration of lacY and galETKM established lactose transport and galactose assimilation via the Leloir pathway. Adaptive laboratory evolution on lactose minimal medium was then employed to enrich mutants with improved growth kinetics. Growth profiling and reverse engineering confirmed mutations critical for lactose metabolism. This approach highlights lactose as both a functional carbon source and a selective pressure in microbial chassis engineering.

Lactose Conversion for One-Pot Catalytic Synthesis of Lactic Acid and HMF

Houndedoke MD, et al. Catalysis Today, 2025, 115658.

This study investigates lactose as a chemical feedstock for platform molecule production via heterogeneous catalysis. Lactose aqueous solutions were subjected to one-pot conversion over Sn-Er/γ-Al₂O₃ catalysts under hydrothermal conditions (170 °C). Experimentally, lactose hydrolysis, sugar isomerization, retro-aldol reactions, and dehydration occurred sequentially within a single reactor. Catalyst synthesis involved metal impregnation followed by calcination, with XRD, XRF, and XPS confirming oxide-phase active sites. Reaction kinetics were evaluated by varying time, temperature, and metal loading, while product yields were quantified chromatographically. Lactose served as the direct reactant enabling lactic acid and HMF formation without enzymatic preprocessing.

Lactose-Driven Biosynthesis of Lacto-N-tetraose in Engineered Escherichia coli

Palur DSK, et al. Metabolic Engineering, 2026, 94, 182-191.

This case study demonstrates lactose as both carbon source and structural precursor for lacto-N-tetraose (LNT) biosynthesis in engineered E. coli. Experimentally, lactose was fed as the sole substrate, while LacZ activity was finely tuned to regulate lactose hydrolysis rates. This controlled generation of glucose and galactose ensured sufficient UDP-sugar precursor supply while retaining intact lactose for glycosylation. Intracellular UDP-sugars were quantified using a dedicated enzymatic assay to guide strain optimization. Fermentation experiments showed efficient conversion of lactose into LNT and LNT II, highlighting lactose's dual experimental role in microbial oligosaccharide synthesis.