Triethoxysilylbutyraldehyde

• CAS: 88276-92-0
• Catalog Number: ACM88276920
• Category: Main Products
• Molecular Weight: 234.36
• Molecular Formula: C10H22O4Si
• Synonyms: 4-(triethoxysilyl)-Butanal
• Boiling Point: 85-71°C
• Appearance: Colorless liquid

Case Study

Triethoxysilylbutyraldehyde for Automated Enzyme Immobilization in High-Throughput Proteomic Workflows

Frey C, et al. Journal of Proteomics, 2024, 303, 105215.

Triethoxysilylbutyraldehyde (TESB) was applied as a silicon-based coupling agent to develop automated workflows for enzyme immobilization in proteomic analyses. TESB and its oxidized derivative, triethoxysilylbutanoic acid (TESBA), demonstrated superior immobilization efficiency (~51 ± 8%) when used to fabricate immobilized enzyme particles (IEPs). These IEPs were produced using an automated liquid handling system (Agilent Bravo), significantly reducing manual workload and enabling simultaneous fabrication of multiple enzyme-coupling agent combinations.
Trypsin-immobilized IEPs using TESB or TESBA provided digestion performance comparable to traditional in-gel methods, but with faster turnaround and enhanced reproducibility. Pepsin IEPs exhibited preferential cleavage at leucine residues (>50%), indicating enzyme-specific substrate selectivity upon immobilization. Furthermore, TESBA was employed to functionalize silica capillary inner walls, enabling the construction of in-situ immobilized enzyme microreactors (IMERs). These IMERs facilitated rapid proteolysis of bovine serum albumin (BSA), achieving 33-40% primary sequence coverage via LC-MS/MS within 15 minutes.