DL-Goitrin

• CAS: 13190-34-6
• Catalog Number: ACM13190346
• Category: Main Products
• Molecular Weight: 129.18
• Molecular Formula: C5H7NOS
• Synonyms: 5-Ethenyl-2-oxazolidinethione; (±)-Goitrin; 5-Vinyl-2-oxazolidine-2-thione; 5-Vinyl-2-oxazolidinethione

Case Study

DL-Goitrin Used in the Study of Expression of Human T2R38 Bitter Taste Receptor

Tran, Hoai TT, et al. Frontiers in immunology, 2018, 9, 2949.

The freshly isolated human peripheral mononuclear blood cells (PBMCs) were used for expression analysis by flow cytometry in order to analyze the expression of the human G protein-coupled bitter taste receptor T2R38 in resting and activated lymphocytes and T cell subsets. The functional relevance of the receptor on lymphocytes was investigated using goitrin in a calcium-based assay. After goitrin treatment, TNF-alpha secretion was quantified in PBMCs with functional and non-functional T2R38 receptors.
Goitrin-related experiments and results
· The effects of the natural T2R38 agonist Goitrin on donor human PBMCs with a functional T2R38 PAV haplotype were investigated by flow cytometry.
Calcium flux was measured in lymphocytes treated with Fluo-4 AM (FEm: 516 nm) and showed a concentration-dependent response to goitrin, beginning at concentrations exceeding 20 μM. At a concentration of 100 μM, goitrin induced a 144% increase in calcium flux compared to the solvent control. Pretreating the cells with the specific PLCβ-2 inhibitor U-73122 prior to the addition of 100 μM goitrin effectively blocked the calcium flux.
· Subsequently, the effect of goitrin on the secretion of the strong pro-inflammatory cytokine TNF-alpha was assessed. In PBMCs expressing either the functional (PAV haplotype) or non-functional (AVI/AVI diplotype) T2R38 receptor, it was observed distinct responses to goitrin. While goitrin had no impact on TNF-alpha secretion in samples with the non-functional T2R38 receptor, it significantly inhibited TNF-alpha release in samples from donors with the functional T2R38, in comparison to the solvent control.

Custom Q&A

What is the molecular formula of DL-Goitrin?

The molecular formula of DL-Goitrin is C5H7NOS.

When was DL-Goitrin created and modified?

DL-Goitrin was created on 2005-03-27 and modified on 2023-12-30.

What is the molecular weight of DL-Goitrin?

The molecular weight of DL-Goitrin is 129.18 g/mol.

What is the IUPAC name of DL-Goitrin?

The IUPAC name of DL-Goitrin is 5-ethenyl-1,3-oxazolidine-2-thione.

What is the InChIKey of DL-Goitrin?

The InChIKey of DL-Goitrin is UZQVYLOFLQICCT-UHFFFAOYSA-N.

How many hydrogen bond donor counts does DL-Goitrin have?

DL-Goitrin has one hydrogen bond donor count.

What is the topological polar surface area of DL-Goitrin?

The topological polar surface area of DL-Goitrin is 53.4 2.

How many heavy atoms does DL-Goitrin contain?

DL-Goitrin contains 8 heavy atoms.

What is the formal charge of DL-Goitrin?

The formal charge of DL-Goitrin is 0.

How many defined atom stereocenter counts does DL-Goitrin have?

DL-Goitrin has 0 defined atom stereocenter counts.