Tetrabutylammonium phosphate

• CAS: 5574-97-0
• Catalog Number: ACM5574970
• Category: Main Products
• Molecular Weight: 339.45
• Molecular Formula: C16H36N.H2O4P
• Synonyms: N,N,N-tributyl-1-butanaminiuphosphate(1:1)
• Melting Point: 151-154 °C
• Appearance: Colorless or white powder
• Storage: 2-8°C

Case Study

Tetrabutylammonium Phosphate as an Ion-Pair Reagent for Enhanced DNA Fragment Separation in Capillary Electrophoresis

Zhang, Sheng, et al. Analytical biochemistry 408.2 (2011): 284-288.

Sensitive and rapid analysis of DNA fragments, particularly for pathogen detection, is essential in biomedical and food safety applications. In this study, tetrabutylammonium phosphate (TBAP) was employed as an additive in a non-gel sieving capillary electrophoresis (NGS-CE) method using hydroxypropylmethylcellulose (HPMC) as the sieving matrix. The tetrabutylammonium cation interacts electrostatically with negatively charged DNA, improving fragment resolution without interfering with UV detection at 260 nm.
Key Results:
· The addition of 15 mM TBAP significantly improved resolution for all DNA fragments (100-1000 bp), with resolution values increasing by 20-40% compared to TBAP-free conditions. Field-enhanced sample injection (FESI) provided 25- to 52-fold sensitivity enhancement, achieving detection limits as low as 2.2-5.8 ng/mL.
· The method successfully separated three multiplex PCR products (from L. monocytogenes, E. coli, and S. enterica) and their corresponding primers in 25 minutes with baseline resolution.
· High reproducibility was demonstrated, with intraday RSDs for migration time and peak area below 2.4% and interday RSDs below 6.1%. PCR product sizes could be accurately estimated using a migration time-based calibration curve, with deviations ≤1.8%.
· TBAP thus serves as an effective, non-absorbing ion-pair reagent that enhances both resolution and sensitivity in DNA analysis by CE-UV, offering a practical tool for diagnostic and food safety applications.

Custom Q&A

What is the molecular formula of Tetrabutylammonium Dihydrogen Phosphate?

The molecular formula of Tetrabutylammonium Dihydrogen Phosphate is C16H38NO4P.

When was Tetrabutylammonium Dihydrogen Phosphate created and last modified?

Tetrabutylammonium Dihydrogen Phosphate was created on 2005-07-19 and last modified on 2023-12-23.

What is the molecular weight of Tetrabutylammonium Dihydrogen Phosphate?

The molecular weight of Tetrabutylammonium Dihydrogen Phosphate is 339.45 g/mol.

What are the synonyms for Tetrabutylammonium Dihydrogen Phosphate?

Some synonyms for Tetrabutylammonium Dihydrogen Phosphate are TETRABUTYLAMMONIUM PHOSPHATE and tetrabutylammonium dihydrogenphosphate.

What are the parent compounds of Tetrabutylammonium Dihydrogen Phosphate?

The parent compounds of Tetrabutylammonium Dihydrogen Phosphate are Tetrabutylammonium and Phosphoric Acid.

What is the InChIKey of Tetrabutylammonium Dihydrogen Phosphate?

The InChIKey of Tetrabutylammonium Dihydrogen Phosphate is ARRNBPCNZJXHRJ-UHFFFAOYSA-M.

What is the hydrogen bond donor count of Tetrabutylammonium Dihydrogen Phosphate?

The hydrogen bond donor count of Tetrabutylammonium Dihydrogen Phosphate is 2.

What is the hydrogen bond acceptor count of Tetrabutylammonium Dihydrogen Phosphate?

The hydrogen bond acceptor count of Tetrabutylammonium Dihydrogen Phosphate is 4.

How many rotatable bonds does Tetrabutylammonium Dihydrogen Phosphate have?

Tetrabutylammonium Dihydrogen Phosphate has 12 rotatable bonds.

What is the topological polar surface area of Tetrabutylammonium Dihydrogen Phosphate?

The topological polar surface area of Tetrabutylammonium Dihydrogen Phosphate is 80.6 Å2.