2'-Fucosyllactose,94%

• CAS: 41263-94-9
• Catalog Number: ALC-FP-41263949
• Category: Featured Products
• Molecular Weight: 488.44
• Molecular Formula: C18H32O15
• Appearance: White to off-white powder
• Ash Content: Max.0.5%
• Content: Min. 94.0%(calculated on the dried basis)
• Difucosyllactose: Max.2.0%
• D-Lactose: Max.3.0%
• Endotoxin Levels: Max.10EU/mg
• Heavy Metal: Total Heavy Metals:Max.10ppm; Arsenic:Max.0.2ppm; Lead:Max.0.05ppm
• Residual Protein: Max.100mg/kg
• Sample Lot No.: A24M0308xMY06
• Total Plate Count: Max.500cfu/g
• Water Content: Max.9.0%

Case Study

Experimental Evaluation of 2'-Fucosyllactose for Modulating Immune Function and Gut Microbiota in Mice

Feng K, et al. Food Bioscience, 2025, 71, 107366.

In this study, 2'-Fucosyllactose (2'-FL), alone and in combination with 6'-sialyllactose (6'-SL), was evaluated for its immunomodulatory and microbiota-modulating effects in BALB/c mice. Mice received oral administration of 2'-FL (low and high doses) or 2'-FL combined with 6'-SL daily for 30 days. Immune responses were quantified through delayed-type hypersensitivity (DTH) tests, carbon clearance assays, macrophage phagocytosis, lymphocyte proliferation, and natural killer (NK) cell activity. Gut microbiota composition was assessed via 16S rRNA sequencing, and short-chain fatty acids (SCFAs) were quantified to evaluate microbial fermentation. Co-supplementation significantly enhanced immune indices and selectively enriched beneficial gut taxa, including Clostridia_UCG_014 and Bacteroides, while increasing SCFA production, demonstrating 2'-FL's functional application as an immune-supportive prebiotic.

Experimental Application of 2'-Fucosyllactose in Modulating Bifidobacterium bifidum Growth and Antibacterial Activity

Du J, et al. Current Research in Food Science, 2025, 10, 100975.

2'-Fucosyllactose (2'-FL) was evaluated for its ability to enhance the probiotic responses of Bifidobacterium bifidum strains YH17 and BBI01. Growth assays demonstrated that the glucose moiety in 2'-FL preferentially fueled biomass accumulation, while galactose was less effective. Gastrointestinal stress models simulated acidic and bile salt conditions, revealing that 2'-FL significantly improved bacterial survival and adhesion. Co-culture experiments with Cronobacter sakazakii ATCC 29544 and Cronobacter muytjensii ATCC 51329 demonstrated that 2'-FL induced strain-specific secretion of thermostable, proteinaceous antibacterial substances, which synergized with medium acidification to inhibit pathogen proliferation. These findings highlight 2'-FL's functional potential in synbiotic formulations and provide a methodological framework for its application in infant gut microbiota modulation.

2'-Fucosyllactose for the Formation of Maillard Reaction Volatiles and α-Dicarbonyl Compounds

Ahn J, et al. Food Research International, 2025, 205, 115975.

This study investigated the experimental application of 2'-fucosyllactose (2'-FL) in Maillard reactions with various amino acids to evaluate volatile and α-dicarbonyl compound (α-DC) formation. Equimolar solutions of 2'-FL (0.1 M) and amino acids, including glycine, threonine, and cysteine, were adjusted to pH 3-9 and heated at 60-150 °C for 30-120 min in 20-mL headspace vials under static conditions. Reaction parameters such as pH, temperature, heating time, and amino acid concentration were systematically varied to assess their influence on α-DC and volatile production. Analytical profiling identified 50 volatiles, with 2'-FL predominantly generating furan derivatives, including 2-furfural, 2-acetylfuran, and furfuryl alcohol. Threonine promoted the highest α-DC formation. These results demonstrate 2'-FL's experimental utility as a flavor precursor and its controlled manipulation in food chemistry applications.

2'-Fucosyllactose for Enhancing Muscle Health in Middle-Aged Mice under Forced Swimming Exercise

Kim JK, et al. Journal of Functional Foods, 2024, 123, 106567.

In this study, 10-month-old male mice were administered 2'-fucosyllactose (2'-FL) concomitant with a regimented forced swimming exercise protocol to evaluate its effects on muscle physiology. Muscle mass, thickness, and strength of the calf, gastrocnemius, and soleus were quantitatively assessed post-intervention. Molecular analyses included qPCR measurement of genes regulating protein synthesis (PI3K, AKT1), hypertrophy (A1R, TRPV4), and degradation (Atrogin-1, MuRF-1), alongside inhibitors of muscle growth (Myostatin, SIRT1). Oxidative stress markers and antioxidant enzyme activity were also evaluated to determine cellular protection. Results demonstrated that 2'-FL significantly enhanced muscle hypertrophy, improved functional strength, and mitigated exercise-induced oxidative damage, highlighting its experimental potential as a dietary supplement to support muscle health.