1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

• CAS: 25952-53-8
• Catalog Number: ACM25952538
• Category: Other Products
• Molecular Weight: 191.7
• Molecular Formula: C₈H₁₈ClN₃
• Synonyms: (3-dimethylaminopropyl)ethyl-carbodiimidmonohydrochloride;3-propanediamine,n'-(ethylcarbonimidoyl)-n,n-dimethyl-monohydrochloride;edap;edc(reagent);WSCD;WSCI;WATER-SOLUBLE CARBODIIMIDE;1-ETHYL-3-(3-DIMETHYLAMINOPROPYL) CARBODIIMIDE
• Melting Point: 110-115°C(lit.)
• Density: 0.877g/mL at 20°C(lit.)
• Hazard Statements: C,Xi
• Safety Description: 26-36/37/39-45-37/39-36
• Supplemental Hazard Statements: H315-H318-H335-H302-H319
• Symbol: GHS05,GHS07

Case Study

N-(3dimethylaminopropyl)-N'-ethylcarbodiimide-Crosslinked Collagen-Chitosan Membranes for Guided Bone Regeneration

Hidalgo-Vicelis, J. L., et al. Fibers and Polymers 21 (2020): 262-272.

Developing biocompatible guided bone regeneration (GBR) membranes requires optimal physicochemical stability (dehydration resistance, mechanical strength, enzymatic degradation resistance) without compromising cellular adhesion or biocompatibility. This study evaluated N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) as a crosslinker for Type I collagen-chitosan (Col I-CS) membranes to enhance material properties while maintaining biocompatibility.
Preparation & Test Approach:
0.5% w/v solutions of Col I and CS (both in 1% acetic acid) were mixed in the following ratios (Col I: CS = 0:100, 25:75, 50:50, 75:25, 100:0), cast as films by solvent evaporation and dried. Membranes were submerged in ≥98% pure EDC solution with agitation for 21 hours, to achieve crosslinking, then rinsed with DI water and dried. Then these membranes characterized using SEM, TGA, DSC, tensile testing, enzymatic degradation assays, and human fetal osteoblast (hFOB) viability/adhesion/morphology studies.
Key Results:
EDC-crosslinked membranes exhibited a rough surface (enhancing cell adhesion) and compact cross-section (barring tissue infiltration). Crosslinking significantly improved dehydration resistance, tensile strength, and enzymatic degradation resistance. All formulations showed no cytotoxicity to hFOBs. The C25 formulation (25% Col I:75% CS) demonstrated optimal mechanical properties for GBR.

EDAC-Crosslinked Ventricular ECM Hydrogels for Cardiac Tissue Engineering

Fujita, Kyohei, et al. AIMS Materials Science 5.1 (2018): 54-74.

The success of hydrogels for cardiac tissue engineering depends on both their biochemical makeup and biomechanical properties recapitulating that of native myocardium. Pure ventricular extracellular matrix (vECM) hydrogels have insufficient equilibrium elasticity and viscoelasticity to match the mechanical properties of decellularized ventricular tissue. In this study, the carbodiimide N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDAC) was used for chemical crosslinking of vECM hydrogels in order to modulate their mechanical properties and maintain the high degree of biocompatibility needed for cardiac tissue engineering applications.
Preparation Methods: First, goat ventricular tissue was decellularized (SDS/Triton X-100), homogenized, and pepsin-digested to form pure vECM solutions (15-22 mg/mL). The solutions were then diluted to concentrations of 7.5/12.5/17.5 mg/mL, cast into molds, and gelled at 37 °C. For EDAC crosslinking, the gels were then submerged in EDAC solutions of various concentrations (10-100 mM) for 24 h at room temperature and rinsed for 48 h.
Key Results:
· Mechanical Tuning: EDAC crosslinking modulated hydrogel properties to match native ventricular tissue in equilibrium elasticity and relaxation coefficient.
· Mechanical enhancement was achievable across EDAC concentrations (10-100 mM) without compromising structural integrity.
· The crosslinked hydrogels also exhibit full cellular compatibility with cells that are relevant to cardiac tissue engineering (fibroblasts, cardiomyocytes), with no negative impact on adhesion or growth.

Custom Q&A

What is the molecular formula of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

The molecular formula of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is C8H18ClN3.

What is the molecular weight of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

The molecular weight of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 191.70 g/mol.

What are some synonyms for 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

Some synonyms for 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride are EDC hydrochloride, EDCI, and N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride.

What is the InChIKey for 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

The InChIKey for 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is FPQQSJJWHUJYPU-UHFFFAOYSA-N.

What is the exact mass of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

The exact mass of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 191.1189253 g/mol.

How many hydrogen bond donor counts are there in 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

There is 1 hydrogen bond donor count in 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride.

What is the topological polar surface area of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

The topological polar surface area of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 28 ².

How many defined atom stereocenter counts are there in 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

There are 0 defined atom stereocenter counts in 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride.

What is the CAS number for 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

The CAS number for 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 25952-53-8.

How many component compounds are present in 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride?

There are 2 component compounds present in 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, which are 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide and Hydrochloric Acid.

Synthetic Use

Upstream Synthesis Route 1

• 628-13-7
• 1892-57-5

• 25952-53-8

Reference: [1] Patent: CN104193654, 2016, B, . Location in patent: Paragraph 0055

Upstream Synthesis Route 2

• 18997-72-3

• 25952-53-8

Reference: [1] Patent: CN104193654, 2016, B,

Downstream Synthesis Route 1

• 25952-53-8

• 1892-57-5

Reference: [1] Patent: US2007/197576, 2007, A1, . Location in patent: Page/Page column 4

Downstream Synthesis Route 2

• 25952-53-8
• 81409-74-7

• 81409-90-7
• 81409-91-8

Reference: [1]Patent: US2008/275240,2008,A1 .Location in patent: Page/Page column 12

* For details of the synthesis route, please refer to the original source to ensure accuracy.