High Performance Liquid Chromatography (HPLC)

HPLC's formidable separation and analytical capabilities and universal applications lay the groundwork for successful operations across diverse industries, particularly in pharmaceutical, nutraceutical, and cosmetic industries.

HPLC, an acronym for high-performance liquid chromatography, is a highly sophisticated technique considered integral in the science and world of analytical chemistry. The system works by the principle of interactions amongst the analytes (compounds to be separated) with both the stationary and mobile phases. HPLC is a variant of column chromatography, but with higher pressure, HPLC results superior efficiency in separating complex mixtures into their constituent parts. This incredible separation efficiency has become a cornerstone of applications ranging from food products to pharmaceuticals and is regularly utilized for an array of purposes, including the purification, quantification, and identification of various components within a mixture.

Fig. 1. Image of high performance liquid chromatograph.

What is HPLC Used For?

In a broad sense, HPLC is employed for the identification, quantification, and separation of various analytes within a mixture. This versatile technique finds its use in a variety of sectors for different purposes, for examples:

Pharma, cosmetic, and dietary products depend heavily on HPLC to ensure their component of products (such as creams, gels, ointments, shampoos, oral suspensions, ophthalmic suspensions, tablets, capsules, and soft gels) are accurately analyzed and safe for consumption.
HPLC is equally significant in environmental labs where it aids in the study of soil, water, and sediment samples.
Additionally, in industrial labs, HPLC helps test any harmful elements in packaging materials, cleaning product ingredients, chemical products, and raw materials, ensuring safety standards are met.
Biological applications of HPLC are equally important, with its use in the analysis of crops, livestock tissues, milk, eggs, blood and urine samples.
In the food industry too, HPLC is crucial, helping ensure the safety and quality of beverages, fruits, vegetables, meat products, baked goods, and other processed items.
Even in other areas like electronics, surfaces can be analyzed using HPLC to check for any harmful residues or substances.

Application of HPLC in Deformulation

HPLC is a commonly used technique in deformulation. By utilizing different columns and detectors, it can be used to analyze the various components present in a complex sample, such as pharmaceuticals, cosmetics, food products, or other formulations. The obtained data can be used to identify the individual components present in a formulation, determine their concentrations, and compare them to known standards or reference materials. This information can help researchers understand the composition of a product, troubleshoot formulation issues, or assess the quality and consistency of a manufactured product. In addition, it can differentiate the active ingredients from the inactive ones, providing key insights into the product formulation's potency, and performance.

Overall, HPLC is a valuable tool in deformulation. By utilizing HPLC in deformulation, researchers can gain valuable insights into the composition of a product and make informed decisions about its formulation, quality, and performance.

How We Can Help You?

Equipped with an experienced team and the latest HPLC systems, Alfa Chemistry offers precise, timely, and personalized deformulation service to our clients from various industries, based on HPLC technique. We have

A stock of more than 200 columns, including those for reverse phase (such as C8 and C18), normal phase (such as hydrophilic interaction liquid chromatography (HILIC)), phenyl-hexyl, cyano and specialty columns for free fatty acids, carbohydrates, proteins, sugars, organic acids, etc.
A number of detection techniques, including ultraviolet/visible absorbance (UV/Vis), photodiode array (PDA), refractive index (RI), evaporative light scattering (ELSD), fluorescence detection (FLD), etc.

Based on these. Whether you are seeking for a better understanding of your own product or a market competitor's offering, we can meticulously decompose and analyse the sample, elucidating on its unique formulation. Our team of specialized scientists, with their profound understanding of HPLC, are ready to assist you in meeting your needs. If there is any need, please feel free to contact us.

Please kindly note that our products and services are for research use only.