Aissaoui N1, Bergaoui L, Boujday S, Lambert JF, Méthivier C, Landoulsi J.
Langmuir. 2014 Apr 15;30(14):4066-77. doi: 10.1021/la404935q. Epub 2014 Apr 2.We investigated the mechanism of enzyme immobilization on silanized surfaces through coupling agents (cross-linkers) in order to understand the role of these molecules on interfacial processes and their effect on catalytic activity. To this end, we used a model multimeric enzyme (G6PDH) and several cross-linking molecules with different chemical properties, including the nature of the end-group (-NCO, -NCS, -CHO), the connecting chain (aliphatic vs aromatic), and geometrical constraints (meta vs para-disubstituted aromatics). Read More