Rock inhibitor y-27632

• CAS: 146986-50-7
• Catalog Number: ACM146986507
• Category: Main Products
• Molecular Weight: 320.26
• Molecular Formula: C₁₄H₂₁N₃O·2HCl
• Synonyms: 4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide
• IUPAC Name: 4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide
• Canonical SMILES: CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N
• InChI Key: IYOZTVGMEWJPKR-VOMCLLRMSA-N
• Boiling Point: 462.6ºC at 760 mmHg
• Flash Point: 233.6ºC
• Density: 1.136 g/cm³
• Appearance: white solid
• Exact Mass: 247.16800
• Hazard Statements: Xn: Harmful

Case Study

Neuroprotective Activity of ROCK Inhibitor Y-27632 during Excitotoxicity

Jeon, Byeong Tak, et al. Neurotoxicity research, 2013, 23, 238-248.

Rho-associated coiled-coil kinase (ROCK) inhibitors protect against neurodegeneration. This work determined the effects of the ROCK inhibitor Y-27632 on neuroprotection after kainate (KA)-induced seizures in mice and during glutamate-induced excitotoxicity in HT22 cells.
Evaluation Methods and Results
· Fluoro-Jade B staining and rapid Golgi staining demonstrated that Y-27632 provides protection against neurodegeneration and neurite dystrophy caused by KA.
· Western blot analysis indicated that Y-27632 reduced the levels of RhoA and ROCK2 in the hippocampus of KA-treated mice.
· Immunohistochemical analysis identified ROCK2-positive neurons and astrocytes in the hippocampus affected by KA.
· Furthermore, in HT22 cells, Y-27632 safeguarded neurons and supported neurite formation during in vitro glutamate-induced excitotoxicity.
· These findings suggested that inhibiting ROCK can influence neurite development and shield neurons from cell death due to excitotoxicity.

Potential of ROCK Inhibitor Y-27632 as Eye Drops for the Treatment of Acute Corneal Endothelial Injury

Okumura, Naoki, et al. Investigative ophthalmology & visual science, 2015, 56(10), 6067-6074.

The aim of this work was to determine if the Rho-associated kinase (ROCK) inhibitor Y-27632 drops can be used to prevent severe corneal endothelial damage after surgical invasion. Result indicated that ROCK inhibitor Y-27632 could be made into eye drops to treat acute corneal endothelial injury to prevent bullous keratopathy.
· Formulation of Y-27632 eye drops
To make Y-27632 eye drops, Y-27632 (10 mM) was prepared by dissolving it in phosphate-buffered saline (PBS; 50 μL) and applying the solution to nine eyes in a corneal endothelial injury model four times daily. Meanwhile, PBS was given at the same frequency to nine control eyes.
· Evaluation methods and results
In the rabbit, Y-27632-treated eyes were observed more Ki67-positive cells than control eyes. Five of the six corneas exposed to Y-27632 had become transparent; none of the six control corneas did (P<0.01). Actin fibers were seen strongly at the cell cortex in Y-27632 eyes while control eyes had partial disruption of actin distribution along with the presence of stress fibers. Expression of N-cadherin and Na+/K+-ATPase was almost all-present in Y-27632 cells, but low in control cells. Preliminary human data showed that ROCK inhibitor eye drops had very promising results in corneal edema after cataract surgery.

Custom Q&A

What is the molecular formula of Rock inhibitor Y-27632?

The molecular formula of Rock inhibitor Y-27632 is C14H23Cl2N3O.

What are the synonyms of Rock inhibitor Y-27632?

The synonyms of Rock inhibitor Y-27632 are Y-27632 dihydrochloride, 129830-38-2, Y-27632 2HCl, and Y 27632 dihydrochloride.

What is the molecular weight of Rock inhibitor Y-27632?

The molecular weight of Rock inhibitor Y-27632 is 320.3 g/mol.

What is the IUPAC name of Rock inhibitor Y-27632?

The IUPAC name of Rock inhibitor Y-27632 is 4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide;dihydrochloride.

What is the InChI of Rock inhibitor Y-27632?

The InChI of Rock inhibitor Y-27632 is InChI=1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11?,12?;;/m1../s1.

What is the InChIKey of Rock inhibitor Y-27632?

The InChIKey of Rock inhibitor Y-27632 is IDDDVXIUIXWAGJ-DDSAHXNVSA-N.

What are the other identifiers of Rock inhibitor Y-27632?

The other identifiers of Rock inhibitor Y-27632 include CAS number 129830-38-2, European Community (EC) number 813-428-5, UNII Q9828II7F3, DSSTox Substance ID DTXSID3042635, Wikidata ID Q27287129, and ChEMBL IDs CHEMBL536267 and CHEMBL1605605.

What is the hydrogen bond donor count of Rock inhibitor Y-27632?

The hydrogen bond donor count of Rock inhibitor Y-27632 is 4.

What is the hydrogen bond acceptor count of Rock inhibitor Y-27632?

The hydrogen bond acceptor count of Rock inhibitor Y-27632 is 3.

What is the topological polar surface area of Rock inhibitor Y-27632?

The topological polar surface area of Rock inhibitor Y-27632 is 68 Ų.