Nucleic acids are water-soluble polyanionic compounds that can combine with many 1- and 2- valent ions to form salts. The latter are neither soluble nor denatured in organic solvents and form precipitates at lower temperatures.
Product List
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| CAS NO. | Product Name | Inquiry |
| 127-09-3 | Sodium acetate | Inquiry |
| 102212-93-1 | Sodium acetate-1-13c-2-d3 | Inquiry |
| 123333-80-2 | Sodium acetate-13c2-2-d3 | Inquiry |
| 13291-89-9 | Sodium acetate-2-(13c) | Inquiry |
| 14044-94-1 | Sodium acetate-d3 | Inquiry |
| 39230-37-0 | Sodium acetate-d3 99+ atom % d | Inquiry |
| 41484-91-7 | Sodium acetate hydrate | Inquiry |
| 56374-56-2 | Sodium acetate-13c2 | Inquiry |
| 66012-98-4 | Sodium acetate-18o2,95 atom % 18o | Inquiry |
| 23424-28-4 | Acetic-1-13c acid,sodium salt(8ci,9ci) | Inquiry |
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Experimental Reagents
1. 3 mol/L sodium acetate buffer, pH 5.2: Weigh 408.1 g of sodium acetate (NaAC·3H2O), dissolve in 800 mL of water, adjust to pH 5.2 with glacial acetic acid, add distilled water to 1000 mL. High pressure sterilization after sub-packing (hereinafter referred to as 3 mol/L sodium acetate buffer).
2．Anhydrous ethanol and 70% (V/V) ethanol.
Experimental Operation
1. Use a pipette to transfer the nucleic acid-containing solution to a new Eppendorf centrifuge tube and measure the volume of the solution.
2. Add 1/10 volume of 3 mol/L sodium acetate buffer. Make the final concentration of sodium acetate 0.3 mol/L.
3. After mixing by inverting, add exactly 2 times the volume (2.5 times the volume for RNA) of pre-cooled absolute ethanol, mix by inverting, and place in an ice bath for 15 to 30 minutes or -20 °C for 30 minutes.
4. Centrifuge at 12000 r/min at 4 °C for 15 min.
5. Hold the Eppendorf at a 45-degree angle so that the nucleic acid precipitate faces upward. Do not touch the precipitate with a micropipette or a tip connected to a vacuum pump.
6. Add pre-cooled 70% ethanol to 2/3 of the volume. Mix and rinse to remove residual salt.
7. Centrifuge at 12000 r/min for 2 minutes at 4 °C, and aspirate the supernatant as in step 5.
8. Leave the tube uncovered and leave it at room temperature for 10-15 minutes or in a vacuum for 2 minutes to allow the ethanol to evaporate.
