10X PBS Recipe Calculator

Phosphate-buffered saline (PBS) is an isotonic buffer solution widely utilized in biological and biochemical research. It maintains pH stability in cell culture, biochemical assays, and molecular biology applications. PBS mimics the ion concentration of the human body, ensuring a stable physiological environment. Alfa Chemistry offers the 10X PBS recipe calculator to simplify buffer preparation and ensure accuracy and reproducibility in biological studies.

The 10X PBS stock solution is a concentrated form, typically diluted to 1X working concentration before use. Calculations can be made using our 1X PBS recipe calculator.

Desired Volume

L

Calculate

Sodium chloride (NaCl)

g

Potassium chloride (KCl)

g

Disodium phosphate dihydrate (Na₂HPO₄·2H₂O)

g

Monopotassium phosphate (KH₂PO₄)

g

Reset

1. Add approximately 80% of the final volume of distilled water to a suitable container.
2. Weigh and add each reagent according to the calculated values.
3. Stir until completely dissolved.
4. Measure the pH and adjust to 7.2–7.4 if necessary using HCl or NaOH.
5. Adjust the final volume with distilled water.
6. Mix thoroughly.
7. Sterilize by filtration (0.22 μm) or autoclaving if required.

Recommended Reagents for PBS Preparation

Chemical	Molecular Weight (g/mol)
Na2HPO4 • 2H2O (ACM7558794)	177.99
KH2PO4 (ACM7778770-3)	136.09
NaCl (ACM7647145)	58.44
KCl (ACM7447407)	74.55

Standard Composition of 10X PBS

A 10X PBS solution is formulated to ensure consistency and reproducibility across experiments. The standard composition includes:

Component	Final Concentration (10X PBS)
NaCl	1.37 M
KCl	27 mM
Na2HPO4	100 mM
KH2PO4	18 mM

Role of Each Component

• Sodium chloride (NaCl): Maintains osmotic balance and physiological ion concentration.
• Potassium chloride (KCl): Supports cell function and maintains electrolyte balance.
• Disodium hydrogen phosphate (Na₂HPO₄) & Monopotassium phosphate (KH₂PO₄): Act as buffering agents, maintaining pH stability (~7.4 at 1X concentration).

Applications of PBS in Research

PBS is indispensable in biological and biochemical research, including:

• Cell Culture: Used for washing cells, diluting solutions, and as a medium component.
• Molecular Biology: Employed in DNA/RNA extractions, immunoassays, and electrophoresis.
• Histology & Microscopy: Used as a rinsing buffer for tissue sample preparation.
• Protein Biochemistry: Applied in Western blotting, ELISA, and protein purification.

Please kindly note that our products are for research use only.