Li D1, Li Q1, Wang S1, Ye J1, Nie H1, Liu Z2.
J Chromatogr A. 2014 Apr 25;1339:103-9. doi: 10.1016/j.chroma.2014.02.084. Epub 2014 Mar 6.Boronate affinity chromatography (BAC) is a unique means for the selective separation and enrichment of 1,2 and 1,3 cis-diol-containing compounds. However, conventional boronate affinity materials require a basic binding pH (usually≥8.5), which gives rise to not only inconvenience in operation but also the risk of degradation of labile compounds. Although the applicable pH has been expanded to 5.0 in recent years, the current boronate affinity materials still fail to meet the acidic pH end of frequently used biosamples, particularly urine (pH 4. Read More