Pseudaminobacter manganicus sp. nov., isolated from sludge of a manganese mine.
Li J1, Huang J2, Liao S3, Wang G4.
Int J Syst Evol Microbiol. 2016 Dec 30. doi: 10.1099/ijsem.0.001765. [Epub ahead of print]
A Gram-staining-negative, aerobic, non-motile, capsule-forming and rod-shaped bacterium, designated JH-7T, was isolated from sludge of a manganese mine. The 16S rRNA gene sequence of strain JH-7T showed highest similarities to Pseudaminobacter salicylatoxidans BN12T (97.4 %), Mesorhizobium thiogangeticum SJTT (97.0 %) and Pseudaminobacter defluvii THI 051T (96.5 %). Phylogenetic trees clustered strain JH-7T together with P. salicylatoxidans BN12T and P. defluvii THI 051T. The DNA-DNA hybridization values between strain JH-7T and P. salicylatoxidans DSM 6986T and between strain JH-7T and M. thiogangeticum DSM 17097T were 34.8 % and 20.1 %, respectively. The major fatty acids of strain JH-7T (> 10 %) were C18:1 ω7c, C19:0 cyclo ω8c and C16:0. The genomic DNA G+C content was 61.6 mol%. The polyamines of strain JH-7T were sym-homospermidine (83 %) and putrescine (17 %), and the respiratory quinone was ubiquinone-10. The major polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids and two unidentified lipids. Compared to the Pseudaminobacter and Mesorhizobium members, strain JH-7T showed some unique physiological and biochemical characters, such as negative in H2S production, hydrolysis of Tween 40 and Tween 60, esterase lipase (C8) activity and assimilation of D-ribose, and positive in acid production from D-galactose and assimilation of D-fructose. On the basis of the polyphasic taxonomic analysis, strain JH-7T was considered to represent a novel species of the genus Pseudaminobacter, for which the name Pseudaminobacter manganicus sp. nov. is proposed. The type strain is JH-7T (= KCTC 52258T = CCTCC AB 2016107T).
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