Polysorbate 80

• CAS: 9005-65-6
• Catalog Number: ALC-FP-9005656
• Category: Featured Products
• Appearance: Light yellow viscous liquid
• Acid Value: 0.5
• Content: Polyoxyethylene:67.6%
• Heavy Metal: ≤ 10 ppm
• Hydroxyl Value: 76
• Ignition Residue: ≤ 0.25%
• Sample Lot No.: A24X02162
• Saponification Value: 49
• Water Content: 1.9% (Karl Fisher)

Case Study

Polysorbate 80 for Stabilization and Immunogenicity of MecVax ETEC Vaccine

Edao B, et al. Vaccine, 2025, 63, 127634.

Polysorbate 80 (0.1 %) was evaluated as a stabilizing excipient for MecVax, a multivalent protein-based vaccine targeting seven ETEC adhesins and two toxins. MecVax antigens, including CFA/I/II/IV MEFA and the toxoid fusion 3xSTaN12S-mnLTR192G/L211A, were formulated with 0.1 % Tween-80, 5 % lactose, or PBS and incubated at 37 °C. Stability assessment via SDS-PAGE showed that CFA/MEFA remained intact for eight weeks in Tween-80 or PBS, whereas toxoid fusion degraded within three weeks, particularly in lactose buffer. Intramuscular immunization of mice with aged formulations induced robust anti-adhesin and antitoxin IgG responses, and sera effectively inhibited ETEC adhesion and toxin activity in vitro, demonstrating polysorbate 80's critical role in enhancing vaccine thermal stability and immunogenicity.

Polysorbate 80 for Brain Delivery of NDP-MSH via Chitosan Nanoparticles

Herrera G, et al. Neuropharmacology, 2024, 253, 109969.

Polysorbate 80 was applied to chitosan nanoparticles (CS NPs) to facilitate brain delivery of α-MSH analog (NDP-MSH). CS solution (0.5 mg/mL) was mixed with PS80 (0.1 mg/mL), heated to 60 °C, and crosslinked with sodium tripolyphosphate at 4 °C under high-speed stirring. NDP-MSH was incorporated during TPP addition to generate [NDP-MSH]-PS80/CS NPs. Fluorescent labeling with FITC allowed tracking of NP uptake. Polysorbate 80 formed a surface layer on NPs, enhancing stability and cellular uptake. This formulation successfully improved brain targeting of NDP-MSH and reversed high-fat diet-induced cognitive impairment, highlighting PS80's role as a surface modifier in nanoparticle-mediated neuropeptide delivery.

Polysorbate 80 for Enhancing Yogurt Bacteria Survival and Adhesion

Ziar H, et al. Saudi Journal of Biological Sciences, 2022, 29(8), 103367.

Polysorbate 80 (1 % w/v) was investigated for its effect on yogurt starter cultures, L. bulgaricus and S. thermophilus. Bacteria were grown in media containing PS80 and exposed to simulated gastric conditions (pH 2.5) with bile salts or cholic acid. PS80 improved bacterial survival under acidic stress, enhanced bile salt hydrolase activity, and promoted cholesterol uptake. Oleic acid incorporation increased membrane unsaturation, and S. thermophilus adhesion to HT-29 intestinal cells increased 2.3-fold. These results demonstrate polysorbate 80's protective effect under digestive stress and its facilitation of bacterial adhesion, supporting its application in probiotic formulations for improved gut colonization and cholesterol metabolism.