Perfluorohexanesulfonic acid

• CAS: 355-46-4
• Catalog Number: ALC-FP-355464
• Category: Featured Products
• Molecular Weight: 400.1
• Molecular Formula: C6HF13O3S
• Sample Lot No.: A23M0705x2404

Case Study

Perfluorohexanesulfonic Acid for Investigating Soil Microbial Interactions and Nitrogen Cycling

Pu Y, et al. Journal of Hazardous Materials, 2025, 498, 139901.

This study evaluated the impact of Perfluorohexanesulfonic acid (PFHxS) on soil microbial communities and functional processes using a controlled microcosm experiment. Soil samples were treated with PFHxS at 10 and 100 μg/kg, followed by high-throughput sequencing to profile rare bacterial, fungal, and protistan communities. PFHxS selectively enriched nirK-type denitrifiers, reshaping microbial interactions and enhancing nitrogen cycling. Network analysis demonstrated that rare microorganisms mediated critical interspecies interactions, which directly influenced soil nitrogen transformations. Additionally, oxidative stress responses were assessed via antioxidant enzyme activity assays, revealing concentration-dependent activation of microbial defense mechanisms. Partial least squares path modeling (PLS-PM) confirmed that alterations in microbial interactions exerted a stronger effect on nitrogen cycling and antioxidant responses than gene abundance or single-community composition. This experimental approach underscores PFHxS's utility as a probe for studying microbial community dynamics, nitrogen metabolism, and defense mechanisms in soil ecosystems.