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Shah, Sajad Hussain, et al. Journal of Plant Growth Regulation 42.12 (2023): 7352-7373.
Gibberellic acid (GA3) is a positive regulator of gibberellin-ABA regulated processes. Below are some examples of the use of GA3 at various stages of plant growth.
· Seed germination: GA3 application is widely reported to break dormancy and trigger germination by way of hormonal regulation and hydrolytic enzyme induction in storage tissues. At the mechanistic level, GA3 stimulates proteasome-mediated RGL2 (DELLA repressor) degradation to permit germination while ABA and other factors such as ABI5 work antagonistically.
· Vegetative growth and development: GA3 applied as foliar sprays, seed priming, or seedling treatments is reported to improve plant height, stem/root elongation, leaf area, biomass and branching across species. Reported examples in the literature include brassica juncea, lycopersicon esculentum and Ixora coccinea, etc.
· Physiological and biochemical effects: Literature evidence suggests that exogenous GA3 application results in improved photosynthetic pigments, photosynthetic rate, carbonic anhydrase (CA) activity, stomatal conductance, protein content and nitrate reductase (NR) activity, etc. Parameters that underpin improved growth and stress tolerance.
· Yield and quality: Field and controlled studies report GA3-mediated increases in flowering, fruit set and yield parameters across horticultural and agronomic crops. For example, in vaccinium virgatum, 500 mg/L GA3 spray led to improved inflorescences, flowers per inflorescence, fertile seeds and fruit weight.
Hamayun, Muhammad, et al. Journal of agricultural and food chemistry 58.12 (2010): 7226-7232.
In this work, exogenous application of gibberellic acid (GA3) was evaluated as a growth stimulant and stress mitigator. Three different concentrations of GA3 (0.5, 1.0 and 5.0 μM) were used to study the dose dependent effect on morphology, biomass, hormone levels and selected metabolites in plants challenged with a single dose of salt.
Experimental setup: A randomized complete block design with eight treatments and three replicates was used in this study. The plants were exposed to four conditions (control, salt stress, GA3 application and GA3+salt stress). Plant growth parameters, phytohormones and phytoestrogens were measured 30 days after sowing.
Key Findings
· Growth recovery: GA3 application significantly increased plant length and fresh/dry biomass relative to salt-only controls, indicating partial or substantial mitigation of NaCl-induced growth suppression. Upon application of GA3 to salt-stressed plants, growth parameters were significantly improved.
· Secondary metabolites: Elevated GA3 treatments raised foliar levels of the isoflavones daidzein and genistein under both control and salt-stress conditions.
· Phytohormones: Treatment with exogenous GA3 led to an increase in the endogenous levels of bioactive gibberellins (GA1 and GA4) and jasmonic acid. Furthermore, it also led to a decrease in ABA and SA. This shift in phytohormone profile is consistent with a reprogramming away from stress-induced dormancy responses toward growth promotion.
· Biosynthetic pathways: Detection of both GA1 and GA4 indicates that soybean utilizes both early C-13-hydroxylation and non-C-13-hydroxylation routes for gibberellin biosynthesis.
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