α-Casein(90-95)

• CAS: 83471-50-5
• Catalog Number: ACM83471505
• Category: Main Products
• Molecular Weight: 783.93
• Molecular Formula: C₃₈H₅₇N₉O₉
• Synonyms: ARG-TYR-LEU-GLY-TYR-LEU;ARG-TYR-LEU-GLY-TYR-LEU-OH;H-ARG-TYR-LEU-GLY-TYR-LEU-OH;ALPHA-CASEIN (90-95);ALPHA-CASEIN FRAGMENT 90-95;A-casein fragment 90-95;fragment90-95;L-Arg-L-Tyr-L-Leu-Gly-L-Tyr-L-Leu-OH
• IUPAC Name: (2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-5-(diaminomethylideneamino)pentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoicacid
• SMILES: CC(C)CC(C(=O)NCC(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CC(C)C)C(=O)O)NC(=O)C(CC2=CC=C(C=C2)O)NC(=O)C(CCCN=C(N)N)N
• InChI Key: KWLNZVXBGCEDOO-QKUYTOGTSA-N
• Density: 1.36g/cm³
• Exact Mass: 783.42800
• WGK Germany: 3

Case Study

α‑Casein(90-95) as a Mast Cell Secretagogue for Evaluating Allergenic Potential of Milk Peptides

Reddi, Srinu, et al. Milchwissenschaft 67.2 (2012): 189-191.

α-Casein(90-95) is a bioactive peptide derived from bovine α-casein. It acts as a non-IgE-dependent mast cell activator, inducing degranulation and release of inflammatory mediators such as histamine and tryptase. This property makes it a useful reference for assessing the allergenicity of food-derived peptides. α-Casein(90-95) is a potent, non-IgE-mediated mast cell activator.
Experimental Protocol: Mast cells were obtained from the peritoneal cavity of rats or differentiated from mouse bone marrow using conditioned medium. Cells were incubated with α-casein(90-95) at milli- to micromolar concentrations. Degranulation was quantified by measuring histamine release (ELISA) and tryptase activity (chromogenic substrate tosyl-Gly-Pro-Lys-p-nitroanilide). Calcium ionophore A23187 served as a positive control.
Performance Evaluation: α-Casein(90-95) elicited a significant secretagogue effect, causing marked histamine and tryptase release from peritoneal mast cells at micromolar levels. In contrast, β-casomorphin-7 required higher concentrations to achieve comparable effects on bone-marrow-derived mast cells. The response was independent of IgE, resembling that of anaphylatoxins. No detectable degranulation was observed in negative controls.