Category
Other Fluorophores
Description
Alfa Chemistry offers Nile Red products for various research purposes. Please contact us by email if you do not find the specification you are looking for on this page.
Synonyms
9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
IUPAC Name
9-(diethylamino)benzo[a]phenoxazin-5-one
Molecular Formula
C20H18N2O2
Canonical SMILES
CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2
InChI
InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChI Key
VOFUROIFQGPCGE-UHFFFAOYSA-N
Appearance
Yellow to Brown to Dark green powder to crystal
Application
Nile red has applications in cell biology, it can be used as a membrane dye, can be easily observed using epifluorescence microscopy, and its excitation and emission wavelengths are often shared with red fluorescent proteins. Nile red has also been used as part of a sensitive detection process for microplastics in bottled water. Furthermore, Nile Red is an excellent candidate for the fabrication of membranes for different sensors to detect environmental changes such as taste, gas, pH, etc.
Absorbance
(E1%1cm) min. 1300(MeOH, 550.0 to 554.0 nm)
Absorption Wavelength
(max.) 552(MeOH) nm
Covalently-Bonded Unit Count
1
Exact Mass
318.136828g/mol
Features And Benefits
Nile red is a lipophilic dye. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile Red does not fluoresce; however, in lipid-rich environments it fluoresces strongly, emitting colors ranging from deep red to deep gold. The dye is highly solvochromic, with emission and excitation wavelengths that vary with solvent polarity, and no fluorescence at all in polar media.
Monoisotopic Mass
318.136828g/mol
Physical State
(20 deg.C) Solid
Storage Conditions
Store at room temperature and dry
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