Confocal microscopy is an optical imaging technique that uses point-by-point illumination and spatial pinhole modulation to remove scattered light from the non-focal plane of the sample, allowing for improved optical resolution and visual contrast compared to traditional imaging methods. Alfa Chemistry, the imaging experts, can provide you with Horiba Raman XploRA Raman Microscope-based confocal microscopy imaging services.
Horiba Raman XploRA Raman Microscope
Technical details
1. Microscope stand: Nikon Eclipse TiU (inverted)
2. Stage: motorized, equipped with Okolab UNO PLUS incubation system
Lasers:
1. 532 nm (Solid State; 100mW)
2. 785 nm (Diode; 90 mW)
3. NDD set: 100%, 50%, 25%, 10%, 1%, 0.1%
4. Gratings: 600 gr/mm, 1200 gr/mm, 1800 gr/mm, 2400 gr/mm
5. Detector: EMCCD (TE cooled); 1600 × 200 pixel; front illuminated
Objectives (default set):
1. Plan Fluor EPI 5×/0.15 Dry, WD=23.5 mm (ZK-5XVIS)
2. Plan Fluor 10×/0.3 Dry, WD=15.2 mm (10×-INV-TiU)
3. Apo Lambda S 40×/1.15 Water, CORR WD=0.6 mm (ZK-40×LWD IM-W-TiU)
4. CFI Plan APO 100×/1.4 Oil, WD=0.13 mm (ZK-100×IM-ÖL-TiU)
5. + Objective for Auto-calibration (all gratings + all laser lines)
The Horiba XploRATM INV Raman is a fully motorized confocal microscope that allows fast non-destructive chemical micro-analysis and automated high definition Raman chemical imaging. The instrument comprises a microscope and a spectrometer built together; this combination enables the acquisition of a complete Raman spectrum at each and every pixel of the image, and then it generates a false colour image based on material composition, phase, crystallinity and strain.
In standard operation this microscope - as any other conventional light microscope -, is diffraction limited, with achievable spatial resolution in the order of 250-300 nm (depending on laser wavelength and objective in use). Features smaller than this can still be detected but without true spatial information.
Standard point-by-point mapping affords the ultimate sensitivity for materials with extremely low Raman scattering properties, and allows high resolution, large spectral range capability but total measurement times can be significant.
Alfa Chemistry' Horiba Raman XploRA Raman Microscope-based confocal microscopy service is an ideal partner for cutting-edge biomedical research, providing precise 3D imaging, as well as accurate imaging of cell structure and dynamic processes, all in one package. If you are interested, please feel free to contact us.
For research use only, not intended for any clinical use.
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