Biological Analysis

Compared to ordinary organic compounds, biomolecules are much bigger in size and molecular weight. There have been only few reports related to the application of click chemistry in biological analysis, most of which focus on the immobilization of enzymes or other biomolecules. In fact, these biomolecules can react with target compounds and produce detectable signal. At present, click chemistry provides a new idea for the construction of this kind of reactions.

Applications

• In foodborne pathogen analysis

Foodborne pathogens in food can cause quite serious and harmful infectious diseases. To ensure food safety, rapid screening and quantitative detection of foodborne pathogens are crucial. Wang et al^[1]. reported a fluorescent/electrochemical dual-signal response biosensing strategy mediated by DNAzyme-ferrocene-triggered click chemistry for the accurate detection of foodborne pathogen Vibrio parahaemolyticus (V.P). As shown in scheme 1, MXene is functionalized to MAADF by gold nanobipyramids (AuNBPs), antimicrobial peptide (AMP), and ferrocene (Fc)-labeled DNAzyme in turn. In the presence of the target, the antibody, V.P, and MAADF form a sandwich complex on the electrode surface. The introduced MAADF acts as the dual-signal output probe towards V.P with the catalytic ability of click reaction and electrochemical activity, which not only triggers the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction between 3-azide-7-hydroxycoumarin (AHC) and 3-butyn-1-ol (BOL) to achieve visual and on-site fluorescent screening of V.P but also realizes the electrochemical response for quantification of V.P.

Scheme 1. (A) Fabrication process of MAADF signal probe; (B) Schematic
illustration of the dual-signal bio-sensor for V.P detection.

• In mycotoxin analysis

Mycotoxins have the potential to contaminate crops and cause disease in humans and animals. Therefore, it is necessary to develop simple and accurate mycotoxin analysis methods. A study described a novel sensitive signal-portable strategy for sensing ochratoxin A (OTA) based on DNA template-mediated click chemistry (Scheme 2)[2]. The personal glucose meter (PGM) background signal of the proposed strategy is greatly improved by using "click" ligation under previously formed dsDNA as a template. Compared with no "click" ligation, the PGM background signal is reduced 7.5 times, and sensitivity and selectivity are greatly promoted.

Scheme 2. Schematic illustration of the portable sensing system.

What Can We Do?

Alfa Chemistry has a strong research foundation in the preparation of chemical sensors for biological analysis. We have ability to help you explore the various applications of click chemistry and provide you with related click chemistry reagents, technical advice and services. If you have problems, please don't hesitate to contact us.

References

1. Wang, S.; et al. Fluorescent/electrochemical dual-signal response biosensing strategy mediated by DNAzyme-ferrocene-triggered click chemistry for simultaneous rapid screening and quantitative detection of Vibrio Parahaemolyticus. Sensors and Actuators: B. Chemical. 2023, 380: 133393.
2. Qiu, S.; et al. DNA template-mediated click chemistry-based portable signal-on sensor for ochratoxin A detection. Food Chemistry. 2019, 297: 124929.