Chicory extract

• CAS: 68650-43-1
• Catalog Number: ACM68650431
• Category: Main Products
• Molecular Weight: 0
• Synonyms: CHICORY EXTRACT;Chicory;Chicory, ext.;CICHORIUM INTYBUS (CHICORY) LEAF EXTRACT;CICHORIUM INTYBUS (CHICORY) ROOT EXTRACT;Chicory extrct;Chicory Root Extract

Case Study

Study of water-soluble chicory extract on perfused jejunum in rats

Kim, Meehye, and Hyun Kyung Shin. The Journal of nutrition 126.9 (1996): 2236-2242.

The direct effects of soluble fibers (water-soluble chicory extract and inulin) on the internal absorption of glucose were studied in enterally perfused rats. After equilibration, jejunal segments and intestinal segments were perfused simultaneously with electrolyte solutions (pH 7.4) containing glucose (7.4 mmol/L) and chicory extract or inulin (10 g/L). Each test or control solution was perfused in a randomized order with a perfusion time of 30 min. Chicory extract or inulin (10 g/L) in the perfusate inhibited jejunal glucose absorption (P < 0.05). The observed changes in glucose and water absorption caused by chicory extract or inulin were reversible after switching to a fiber-free perfusate. Moreover, the net water absorption became secretory after the addition of chicory extract or inulin.
In each rat, the jejunum and lysate were perfused independently and simultaneously using an in situ perfusion technique. After an initial perfusion of 10 min with saline, both segments were fused with an isotonic electrolyte solution (pH 1) (Table 10) containing glucose (7.4 mmol/L) and chicory extract or inulin (10 g/L). The following applied to each perfused segment: the cannula from the intestinal loop was connected to a reservoir of perfusion solution maintained at 37°C via Teflon tubing; a peristaltic perfusion pump recirculated the solution through the intestinal loop at a flow rate of 0.5 mL/min (a rate selected based on preliminary experience); the perfusion solution was gassed with O2 95%:CO2 5% (v/v) and also contained 5 g/L polyethylene glycol (PEG) 4000 as a nonabsorbable marker for fluid transport. In the experiment, 5 rats were perfused with each test article and control solution.

Chicory extracts investigated as potential antifungal agents

Mares, Donatella, et al. Mycopathologia 160 (2005): 85-91.

Chicory extracts were investigated for possible biological activity against fungi from various ecological contexts: some are parasites on plants (phytopathogens) or animals and humans (zoophilic and anthropophilic dermatophytes), others are parasitic on soil and rarely parasitic on animals (geophilic dermatophytes). The extracts were ineffective against geophilic species and the tested phytopathogens, with the exception of Pythium ultimum, while they inhibited the growth of zoophilic and anthropophilic dermatophytes, in particular Trichophyton tonsurans var. sulfureum, whose treatment resulted in morphological abnormalities, observed here by scanning electron microscopy.
Chicory extracts were filtered on a filter and then added to the culture medium. The amount of guaiacolactones in the extracts was determined by chromatographic analysis equipped with a scanner. For the experiments, small mycelial disks of the fungi were taken from the mother culture, grown on appropriate media (SDA for dermatophytes and PDA for phytopathogens) and placed in Petri dishes containing free medium at 26 ± 2 °C until they reached the mid-logarithmic phase of growth. They were then transferred to dishes containing chicory extract at the above doses (1%, 2%, 4% and 20%). Only on T. tonsurans (the most inhibited fungus), both guaiacolactones were tested at a single concentration of 10 lg/ml; this dose corresponds to the 4% and 20% extract treatments of 8-deoxylactate.

Chicory extract inhibits cyclooxygenase-2 expression and activity

Cavin, C., et al. Biochemical and biophysical research communications 327.3 (2005): 742-749.

Chicory is a major source of fructans reported to possess prebiotic-bifidogenic properties. In the present study, the potential anti-inflammatory activity of chicory was investigated. Chicory root extract significantly inhibited prostaglandin E (PGE) production in human colon cancer HT29 cells treated with the pro-inflammatory agent TNF-a. Two independent mechanisms of action were identified: (1) strong inhibition of TNF-a-induced cyclooxygenase 2 (COX-2) protein expression and (2) direct inhibition of COX enzyme activity with significant selectivity for COX-2 activity. The major sesquiterpene lactone of chicory root, guaiacolactone 8-deoxylactate, was identified as the key inhibitor of COX-2 protein expression present in chicory extract. The data presented strongly support that chicory root is a promising source of functional food ingredient combining prebiotic and anti-inflammatory properties.
Treatment with chicory extract or 8-deoxylactose for 1 h followed by treatment in the presence of the proinflammatory agent TNF-a (10 lg/ml) for an additional 6 h. Nuclear proteins were extracted from HT29 cells by addition of cold hypotonic buffer. After incubation on ice for 30 min, the cells were pelleted by centrifugation at 3000 g for 30 min at 4 °C. The supernatant formed the cytosolic fraction and the pellet was resuspended in cold hypertonic buffer and centrifuged. Cell debris was removed by centrifugation at 12,000 g for 30 min at 4 °C. The supernatant fraction containing nuclear proteins was stored at 80 °C. Binding reactions were performed on ice for 40 min with nuclear proteins in binding buffer and 30,000 cpm of P-labeled oligonucleotides labeled with T4 polynucleotide kinase and [cP]ATP. DNA-protein complexes were separated from unbound DNA probes on 5% polyacrylamide gels.

Effect of chicory extract on immunomodulatory activity

Saybel, O. L., et al. Pharmacognosy journal 12.5 (2020).

The immunomodulatory activity of chicory extract was studied and the prospects for drug development were evaluated based on it. The immunomodulatory effect of chicory extract has been explored in in vivo experiments involving intact animals treated with azathioprine cytostatics as well as immunosuppressed animals. The effect of chicory extract on the status of the cellular immune component was evaluated in delayed hypersensitivity reactions. The status of humoral immunity was evaluated by counting antibody-forming cells determined by local hemolysis. The status of the macrophage component of the immune response was evaluated in the phagocytic reaction of peritoneal macrophages associated with colloidal liquid ink particles. Chicory extract was able to reduce the inhibitory effect of azathioprine on cell-mediated immune responses, antibody responses, and macrophage phagocytosis; it did not alter the immune parameters in intact animals.
Chicory extract was administered to intact mice in the third experimental group at a dose of 30 mg/kg/orally once daily for 14 days. The experimental therapeutic dose of dry chicory extract equal to 30 mg/kg was empirically determined in preliminary tests involving 30 mice. As a reference drug, Immunal (Lec Pharma, Slovenia) was used. Mice of experimental group 2 and intact mice of experimental group 4 were immunized with an equieffective dose of 5 ml/kg once a day on a background of azathioprine for 14 days. Intact and control groups of animals received purified water according to a similar treatment schedule.