5-Methoxyuridine

• CAS: 35542-01-9
• Catalog Number: ACM35542019
• Category: Main Products
• Molecular Weight: 274.23
• Molecular Formula: C₁₀H₁₄N₂O₇
• Synonyms: 5-OMe-uridine; 5-METHOXYURIDINE
• IUPAC Name: 1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methoxypyrimidine-2,4-dione
• SMILES: COC1=CN(C(=O)NC1=O)C2C(C(C(O2)CO)O)O
• InChI Key: ZXIATBNUWJBBGT-JXOAFFINSA-N
• Appearance: solid
• EC Number: 252-609-8
• Exact Mass: 274.08000

Case Study

5-Methoxyuridine for Enhanced mRNA Transfection via PBAE-PEG/LNP in Lung Cell-Specific Gene Delivery

Liu B, et al. Molecular Therapy, 2025, 33(3), 1154-1165.

In a recent study on lung-targeted gene delivery, 5-Methoxyuridine-modified mRNA was employed to improve transfection efficiency in vivo. Researchers formulated lipid nanoparticles (LNPs) comprising poly(β-amino ester)-PEG (PBAE-PEG), 4A3-SC8, DOPE, cholesterol, and DOTAP to encapsulate modified mRNA. Intravenous administration of PBAE-PEG/LNPs achieved 73% transfection efficiency in lung endothelial cells, while intratracheal delivery attained 55% in alveolar type II epithelial cells in mice. Comparatively, PBAE/LNPs demonstrated superior specificity for airway club epithelial cells via intratracheal injection. The study revealed that 5-Methoxyuridine modification enhanced mRNA translation in vivo but not in vitro, highlighting its functional advantage in biological systems. Lipofectamine MessengerMAX, although effective in vitro, showed no in vivo activity. This work underscores the utility of 5-Methoxyuridine-modified mRNA in combination with tailored LNP formulations for efficient and cell-specific gene delivery to the lung, advancing therapeutic strategies for genetic pulmonary diseases.

Custom Q&A

What is the molecular formula of 5-Methoxyuridine?

The molecular formula of 5-Methoxyuridine is C10H14N2O7.

What is the molecular weight of 5-Methoxyuridine?

The molecular weight of 5-Methoxyuridine is 274.23 g/mol.

What is the IUPAC name of 5-Methoxyuridine?

The IUPAC name of 5-Methoxyuridine is 1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methoxypyrimidine-2,4-dione.

What is the InChI of 5-Methoxyuridine?

The InChI of 5-Methoxyuridine is InChI=1S/C10H14N2O7/c1-18-4-2-12(10(17)11-8(4)16)9-7(15)6(14)5(3-13)19-9/h2,5-7,9,13-15H,3H2,1H3,(H,11,16,17)/t5-,6-,7-,9-/m1/s1.

What is the InChIKey of 5-Methoxyuridine?

The InChIKey of 5-Methoxyuridine is ZXIATBNUWJBBGT-JXOAFFINSA-N.

What are the synonyms of 5-Methoxyuridine?

The synonyms of 5-Methoxyuridine are Uridine, 5-methoxy-, mo(5)u, and 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methoxypyrimidine-2,4(1H,3H)-dione.

What is the CAS number of 5-Methoxyuridine?

The CAS number of 5-Methoxyuridine is 35542-01-9.

What is the European Community (EC) Number of 5-Methoxyuridine?

The European Community (EC) Number of 5-Methoxyuridine is 252-609-8.

What is the XLogP3 value of 5-Methoxyuridine?

The XLogP3 value of 5-Methoxyuridine is -1.8.

How many hydrogen bond donor counts does 5-Methoxyuridine have?

5-Methoxyuridine has 4 hydrogen bond donor counts.