Photoinduced Charge Transfer Dynamics in A Guanine and Thymine Dinucleotide Model
Duchi M, et al. Physical Chemistry Chemical Physics, 2019, 21(26), 14407-14417.
The main nonradiative relaxation dynamics of guanine and thymine model nucleotides (2'-deoxyguanosine 3'-monophosphate 5'-thymidine, d(GpT)) in buffered aqueous solutions were studied. Results show that the Franck-Condon excited state of d(GpT) is significantly delocalized at both nucleobases and mediates the d(G+ pT- ) exciplex product on ultrafast (<350 fs) time scales formation. Exciplexes rapidly convert back to the neutral ground state molecule on a time scale of ~10 ps with high quantum yield, ensuring the photostability of the nucleotide sequence. Preparation of d(GpT) · Using 2'-deoxyguanosine-5'-monophosphate disodium salt (>98%) and thymidine-5'-monophosphate disodium salt (>99%) as raw materials, standard phosphoramidite coupling and deprotection procedure for the preparation of 2'-deoxyguanosine 3'-monophosphate 5'-thymidine. The final compound was purified by preparative HPLC. · For all reported spectroscopic studies, mono- and di-nucleotides were prepared at 10 mM concentration in D2O phosphate buffer solution (pD 7).
Application of 2'-Deoxyguanosine 3'-Monophosphate in the Synthesis of Guanine Quartets
Stefan L, et al. Nature Reviews Chemistry, 2019, 3(11), 650-668.
Guanine and related nucleobases such as guanosine, deoxyguanosine, and isoguanosine are well-known molecular tools for designing functional supramolecular assemblies. For example, 2'-deoxyguanosine-3'-monophosphate can be used to synthesize lipophilic and water-soluble G-quartets. Sherman and colleagues scrutinized the structure of tetrameric GMP, following the lipophilic TASQ approach and exploiting the phosphate group of the 2'-deoxyguanosine-3'-monophosphate to link guanines to the cavitand template. Lipophilic versus water-soluble G-quartets · LipoGs have been instrumental in understanding the factors that govern G-quartet formation and stability, thanks to the broad variety of techniques implementable in organic solvents. · From a structural point of view, the simplest hydrophilic monomer is guanosine monophosphate. Early investigations on G-quartet formation were performed using concentrated GMP solutions (25mg·mL-1). The structure of GMP-based, hydrophilic G-quartets was further investigated later on, through modern analytical techniques. · The presence of cations is not mandatory for the G-quartet formation of water-soluble TASQ; this new molecular tool was not exploited for cation chelation but as a DNA-mimicking platform to investigate the interactions between G-quartets and G4-ligands.
※ Please kindly note that our products are for research use only.