Crane JM1, Frodyma ME, Bergstrom GC.
J Appl Microbiol. 2014 Jun;116(6):1572-83. doi: 10.1111/jam.12480. Epub 2014 Mar 17.AIMS: In this study, we investigated the feasibility of applying nutrient germinants to plant surfaces to stimulate the spore germination of the plant disease biocontrol agent Bacillus amyloliquefaciens strain TrigoCor.METHODS AND RESULTS: Using the terbium chloride assay and phase-contrast microscopy, we screened potential germinants of TrigoCor spores and found that a combination of d-glucose, d-fructose and potassium chloride (GFK), in addition to either l-asparagine (Asn-GFK) or l-alanine (Ala-GFK), induced maximal levels of TrigoCor spore germination in vitro. Read More