Panferov VG1, Safenkova IV1, Varitsev YA2, Drenova NV3, Kornev KP3, Zherdev AV1, Dzantiev BB1.
Talanta. 2016 May 15;152:521-30. doi: 10.1016/j.talanta.2016.02.050. Epub 2016 Feb 23.Ralstonia solanacearum is a dangerous and economically important pathogen of potatoes and other agricultural crops. Therefore, rapid and sensitive methods for its routine diagnostics are necessary. The aim of this study was to develop a rapid control method for R. solanacearum with a low limit of detection (LOD) based on a lateral flow immunoassay (LFIA) with silver enhancement. To minimize the LOD, the membrane type, antibody amount for conjugation with gold nanoparticles, conjugate concentration and antibody concentration in the analytical zone were optimized. Read More