Zhu C1, Ma X1, Wang Y1, Mi Y2, Fan D3, Deng J1, Xue W4.
Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:411-6. doi: 10.1016/j.msec.2014.08.010. Epub 2014 Aug 10.To improve zinc binding ability to human-like collagen (HLC) and stability of metal complex, HLC was thiolated by mercaptosuccinylation reaction with S-acetylmercaptosuccinic anhydride (S-AMSA) at pH8.0. One mole of thiolated HLC-Zn (SHLC-Zn) complex possessed 24.3mol zinc ions when pH was 8.0 and zinc concentration was 15 mM. The physicochemical properties and biocompatibility of thiolated HLC-Zn (SHLC-Zn) complex were investigated by UV-vis, CD, electrophoresis analysis, differential scanning calorimetry (DSC) and cell viability assay, respectively. Read More