Montes MR1, Ferreira-Gomes MS2, Centeno M2, Rossi RC2.
Biochim Biophys Acta. 2015 Jul;1848(7):1514-23. doi: 10.1016/j.bbamem.2015.03.023. Epub 2015 Mar 30.The first X-ray crystal structures of the Na,K-ATPase were obtained in the presence of magnesium and fluoride as E2(K2)Mg-MgF4, an E2∙Pi-like state capable to occlude K(+) (or Rb(+)). This work presents a functional characterization of the crystallized form of the enzyme and proposes a model to explain the interaction between magnesium, fluoride and Rb(+) with the Na,K-ATPase. We studied the effect of magnesium and magnesium fluoride complexes on the E1-E2 conformational transition and the kinetics of Rb(+) exchange between the medium and the E2(Rb2)Mg-MgF4 state. Read More