Yun CH1, Bae CS2, Ahn T3.
Protein Expr Purif. 2016 Nov;127:68-72. doi: 10.1016/j.pep.2016.07.006. Epub 2016 Jul 12.We investigated a "one-step" method for transformation of and protein expression in Escherichia coli (E. coli) using a complex of n-stearylamine, a cationic lipid, and plasmid DNA, which mimics lipoplex-based approaches. When E. coli cells were treated with the cationic lipid-plasmid complex, the transformation efficiencies were in the range of approximately 2-3 × 10(6) colony-forming units. Further increase in the efficiency was obtained by co-treatment with calcium chloride (or rubidium chloride) and the complexes. Read More