Ding J1, Wang X, Qin W.
ACS Appl Mater Interfaces. 2013 Oct 9;5(19):9488-93. doi: 10.1021/am402245f. Epub 2013 Sep 24.Pulsed galvanostatic control of ion fluxes across polymeric membrane ion-selective electrodes (ISEs) is an emerging field for potentiometric sensing. Herein we report a novel potentiometric enzyme immunoassay based on current-controlled release of an enzyme substrate, which eliminates the addition of marker ions in the sample solution. In this method, the carboxylated poly(vinyl chloride) matrix at the outer layer of the ISE membrane is employed to attach a primary antibody. Read More