Müller WE1, Tolba E2, Ackermann M3, Neufurth M2, Wang S2, Feng Q4, Schröder HC2, Wang X5.
Acta Biomater. 2016 Dec 22. pii: S1742-7061(16)30714-0. doi: 10.1016/j.actbio.2016.12.045. [Epub ahead of print]Here we describe the fabrication process of amorphous strontium-polyphosphate microparticles ("Sr-a-polyP-MP"). The effects of these particles on growth and gene expression were investigated with SaOS-2 cells as well as with human mesenchymal stem cells (MSC) and compared with those particles prepared of amorphous calcium-polyphosphate ("Ca-a-polyP-MP") and of strontium salt. The results revealed a markedly higher stimulation of growth of MSC by "Sr-a-polyP-MP" compared to "Ca-a-polyP-MP" and a significant increase in mineralization of SaOS-2 cells, as well as an enhanced upregulation of the expression of the genes encoding for alkaline phosphatase and the bone morphogenetic protein 2 (BMP-2), likewise performed with SaOS-2 cells. Read More