Jiménez A1, Zhang J, Matthews MA.
Biotechnol Bioeng. 2008 Dec 15;101(6):1344-52. doi: 10.1002/bit.21983.The purpose of the present work is to evaluate a novel CO(2)-based cold sterilization process in terms of both its killing efficiency and its effects on the physical properties of a model hydrogel, poly(acrylic acid-co-acrylamide) potassium salt. Suspensions of Staphylococcus aureus and Escherichia coli were prepared for hydration and inoculation of the gel. The hydrogels were treated with supercritical CO(2) (40 degrees C, 27. Read More