Zhao L1, Chen H, Chen D, Hu YP, Yu H, Wang QQ, Tang GP, Hu XR.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2009 Jan;38(1):59-66.OBJECTIVE: To construct a drug carrier and gene vector PEG-PEI-Pt.METHODS: Polyethyleneglycol (PEG) was coupled to polyethylenimine (PEI 600) and platinum tetrachloride; PEG-PEI-Pt complex was formed in ethanol. The complex was characterized by XRD, UV-VIS and FT-IR and the DNA condensation was tested by electrophoretic mobility shift assay. The cell viability was evaluated by MTT assay in Hela, B16, A293 and COS-7 cells and in vitro transfection efficiency was measured in A293 and B16 cells. Read More