Ortolani F1, Petrelli L, Nori SL, Gerosa G, Spina M, Marchini M.
Histol Histopathol. 2003 Oct;18(4):1131-40.Subdermal implant models are helpful in the study of calcification "in vivo" and for testing anticalcific treatments. After implantation of porcine aortic valve leaflets in rat subcutis, we previously found that glutaraldehyde-Cuprolinic blue reactions (GA-CB) at low pH induce favourable tissue unmasking from mineral deposits, and visualize peculiar, electrondense layers that outline the calcifying cells and matrix vesicle-like structures. Read More