Gihaz S1, Weiser D2,3,4, Dror A1, Sátorhelyi P3, Jerabek-Willemsen M5, Poppe L2,4,6, Fishman A1.
ChemSusChem. 2016 Nov 23;9(22):3161-3170. doi: 10.1002/cssc.201601158. Epub 2016 Oct 25.Two ternary sol-gel matrices, an octyltriethoxysilane-based aliphatic matrix and a phenyltriethoxysilane (PTEOS)-based aromatic matrix, were used to immobilize a methanol-stable variant of lipase from Geobacillus stearothermophilus T6 for the synthesis of biodiesel from waste oil. Superior thermal stability of the mutant versus the wildtype in methanol was confirmed by intrinsic protein fluorescence measurements. Read More