Luo C1,2, Lü D2, Pan J1, Long M2.
ACS Appl Mater Interfaces. 2016 Apr 6;8(13):8367-75. doi: 10.1021/acsami.6b00353. Epub 2016 Mar 23.Manipulation of genes in human embryonic stem cells (hESCs) is imperative for their highly potential applications; however, the transduction efficiency remains very low. Although existing evidence revealed the type, size, and zeta potential of vector affect gene transfection efficiency in cells, the systematic study in hESCs is scarce. In this study, using poly(amidoamine) (PAMAM) dendrimers ended with amine, hydroxyl, or carboxyl as model, we tested the influences of size and surface group as well as cytotoxicity and endocytosis on hESC gene transfection. Read More