Ursuegui S1, Yougnia R, Moutin S, Burr A, Fossey C, Cailly T, Laayoun A, Laurent A, Fabis F.
Org Biomol Chem. 2015 Mar 28;13(12):3625-32. doi: 10.1039/c4ob02636e.Isatoic anhydride derivatives, including a biotin and a disulfide linker were specifically designed for nucleic acid separation. 2'-OH selective RNA acylation, capture of biotinylated RNA adducts by streptavidin-coated magnetic beads and disulfide chemical cleavage led to isolation of highly enriched RNA samples from an initial 9/1 DNA-RNA mixture. Starting from the parent compound N-methylisatoic anhydride A which was used at 65 °C, we improved the extraction process by designing a new generation of isatoic anhydrides that are able to react under smoother conditions. Read More