Description
Acid Brown 4 is a widely used chemical reagent in laboratory experiments. It has been used for many years in biochemical, physiological, and medical research.
Synonyms
Acid Brown 4;Acid brown 4 (C.I. 14805)
IUPAC Name
sodium;6-[(4-aminophenyl)diazenyl]-5-hydroxynaphthalene-1-sulfonate
Molecular Formula
C16H12N3O4SNa
Canonical SMILES
C1=CC2=C(C=CC(=C2O)N=NC3=CC=C(C=C3)N)C(=C1)S(=O)(=O)[O-].[Na+]
InChI
InChI=1S/C16H13N3O4S.Na/c17-10-4-6-11(7-5-10)18-19-14-9-8-12-13(16(14)20)2-1-3-15(12)24(21,22)23;/h1-9,20H,17H2,(H,21,22,23);/q;+1/p-1
InChI Key
VFYDFVDOOIPQHX-UHFFFAOYSA-M
Solubility
Soluble in water, ethanol and concentrated sulfuric acid.
Application
Acid Brown 4 is used in a variety of scientific research applications. It is used as an enzyme inhibitor, a fluorescent probe, a chromogenic reagent, and a fluorescent label. It has also been used in immunological assays, in the study of protein-protein interactions, and in the study of DNA-protein interactions.
Features And Benefits
Soluble in water for red light brown, soluble in ethanol for yellow light brown. The strong sulfuric acid for wine red, dark blue, diluted to the red-orange. Strong hydrochloric acid solution to join a brown orange precipitation; Add thick sodium hydroxide solution for palm orange.