3-azido-L-tyrosine

Tub-tag labeling; chemoenzymatic incorporation of unnatural amino acids

Helma J, Leonhardt H, Hackenberger C P R, et al

Noncanonical Amino Acids Humana Press, New York, NY, 2018: 67-93

Tub-tag labeling is a chemoenzymatic method that enables the site-specific labeling of proteins. Here, the natural enzyme tubulin tyrosine ligase incorporates noncanonical tyrosine derivatives to the terminal carboxylic acid of proteins containing a 14-amino acid recognition sequence called Tub-tag. The tyrosine derivative carries a unique chemical reporter allowing for a subsequent bioorthogonal modification of proteins with a great variety of probes. Here, we describe the Tub-tag protein modification protocol in detail and explain its utilization to generate labeled proteins for advanced app...