Li G1, Ren J, Iwaki H, Zhang D, Hasegawa Y, Wu Q, Feng J, Lau PC, Zhu D.
Appl Microbiol Biotechnol. 2014 Feb;98(4):1681-9. doi: 10.1007/s00253-013-5028-1. Epub 2013 Jun 23.A cyclohexylamine oxidase (CHAO) of bacterial origin was previously shown to be a potentially useful catalyst in the deracemization of racemic primary amines. To further explore the properties and application of this enzyme, five single-amino acid substitution mutants (L199A, M226A, Y321A, Y321F, and L353M) were created based on superimposition of the tertiary structure of CHAO and the monoamine oxidase (MAO) B homolog. Read More