Sheedy JR1, Ebeling PR, Gooley PR, McConville MJ.
Anal Biochem. 2010 Mar 15;398(2):263-5. doi: 10.1016/j.ab.2009.11.027. Epub 2009 Nov 23.We describe a general protocol for preparing protein-containing biofluids for (1)H nuclear magnetic resonance (NMR) metabolomic studies. In this protocol, untreated samples are diluted in deuterated solvents to precipitate proteins and recover metabolites quantitated relative to standard reference compounds such as 3-trimethylsilylpropionic acid (TSP) and 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS). Read More