Sterzynska K1, Budna J, Frydrych-Tomczak E, Hreczycho G, Malinska A, Maciejewski H, Zabel M.
Folia Histochem Cytobiol. 2014;52(3):250-5. doi: 10.5603/FHC.2014.0029.The immobilization of antibodies on various surfaces has been the subject of advanced research in various immunoassay-based diagnostic devices. The physical and chemical stabilities of the immobilized antibodies on a solid surface still cause many problems because upon immobilizing antibody molecules, the antigen-binding ability usually decreases. The silanization of surfaces with organosilanes carrying chemically active groups such as (3-aminopropyl) triethoxysilane (APTES) can accommodate these antigen-binding molecules in an appropriate orientation so that their functionality and binding activity are essentially retained. Read More