Li GW1,2, Zhang Y1,2, Li YP1,2, Wu JX1,2, Xu XL1,2.
Arch Insect Biochem Physiol. 2016 Feb;91(2):67-87. doi: 10.1002/arch.21309. Epub 2015 Nov 26.Odorant-binding proteins (OBPs) act in insect olfactory processes. OBPs are expressed in the olfactory organs and serve in binding and transport of hydrophobic odorants through the sensillum lymph to olfactory receptor neurons within the antennal sensilla. In this study, three OBP genes were cloned from the antennal transcriptome database of Grapholita molesta via reverse-transcription PCR. Recombinant GmolOBPs (rGmolOBPs) were expressed in a prokaryotic expression system and enriched via Ni ion affinity chromatography. Read More