Zarate E1, Boyle V2, Rupprecht U3, Green S4, Villas-Boas SG5, Baker P6,7, Pinu FR8.
Metabolites. 2016 Dec 29;7(1). pii: E1. doi: 10.3390/metabo7010001.Gas Chromatography-Mass Spectrometry (GC-MS) has long been used for metabolite profiling of a wide range of biological samples. Many derivatisation protocols are already available and among these, trimethylsilyl (TMS) derivatisation is one of the most widely used in metabolomics. However, most TMS methods rely on off-line derivatisation prior to GC-MS analysis. In the case of manual off-line TMS derivatisation, the derivative created is unstable, so reduction in recoveries occurs over time. Read More